Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

ELISA: Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence) [NBP2-66682] - Samples were spiked with high concentrations of Human Autoimmune Regulator/AIRE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence) [NBP2-66682] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL

Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence) Summary

• Specificity: This kit recognizes Human AIRE in samples. No significant cross-reactivity or interference between Human AIRE and analogues was observed.
• Standard Curve Range: 31.25-2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-CLIA
• Inter-Assay: CV% < 9.77%
• Intra-Assay: CV% < 9.66%
• Spike Recovery: 91-106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Chemiluminescence)
• Gene: AIRE

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Autoimmune Regulator/AIRE ELISA Kit (Chemiluminescence)

• AIRE
• APECED protein
• APECED
• APECEDAPSI
• APS1
• APSI
• Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein
• autoimmune regulator (APECED protein)10APS1AIRE1
• autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy)
• Autoimmune Regulator
• PGA1

Background

AIRE encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Autoimmune Regulator/AIRE ELISA Kit (NBP2-66682). (Showing 1 - 1 of 1 FAQ).

1. I am looking into a positive control for Western blotting. We are trying to detect the AIRE protein and are not sure whether the antibody we have is picking up the protein. Are you able to tell me whether the human thymus whole tissue lysate (NB820-59456) has been used to detect for AIRE expression by Western blotting?
   • I am not currently aware of any references to the use of our human thymus whole tissue lysate being used to detect AIRE by Western blotting. On looking through the Western blot images for all seven of our AIRE antibodies, I see that these have been generated using lysates from RT-4 cells (bladder), U-251 MG cells (brain), 293 cells (human embryonic kidney), human spleen lysate, liver lysate and tonsil lysate. Additionally, you may find the Human Protein Atlas link to AIRE useful. At this site you can find protein expression profiles based on immunohistochemistry for a large number of human tissues, cancers and cell lines: The Human Protein Atlas.

Bioinformatics

• Gene Symbol: AIRE