Autoimmune Regulator/AIRE Antibody (609930) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB, CyTOF-ready, Flow
• Clone: 609930
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

Autoimmune Regulator/AIRE Antibody (609930) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant mouse AIRE
  Ser476-Ser552 (predicted)
  Accession # Q9Z0E3
• Specificity: Detects mouse AIRE in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human AIRE is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: AIRE
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Autoimmune Regulator/AIRE Antibody (609930) [Alexa Fluor® 532]

• AIRE
• APECED protein
• APECED
• APECEDAPSI
• APS1
• APSI
• Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein
• autoimmune regulator (APECED protein)10APS1AIRE1
• autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermaldystrophy)
• Autoimmune Regulator
• PGA1

Background

AIRE encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CBP. At least three splice variant mRNAs products have been described including one which results in a premature stop codon and a transcript predicted to be a candidate for nuclear-mediated decay (NMD). Defects in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy-candidiasis-ectodermal dystrophy (APECED).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Autoimmune Regulator/AIRE Antibody (FAB6184X). (Showing 1 - 1 of 1 FAQ).

1. I am looking into a positive control for Western blotting. We are trying to detect the AIRE protein and are not sure whether the antibody we have is picking up the protein. Are you able to tell me whether the human thymus whole tissue lysate (NB820-59456) has been used to detect for AIRE expression by Western blotting?
   • I am not currently aware of any references to the use of our human thymus whole tissue lysate being used to detect AIRE by Western blotting. On looking through the Western blot images for all seven of our AIRE antibodies, I see that these have been generated using lysates from RT-4 cells (bladder), U-251 MG cells (brain), 293 cells (human embryonic kidney), human spleen lysate, liver lysate and tonsil lysate. Additionally, you may find the Human Protein Atlas link to AIRE useful. At this site you can find protein expression profiles based on immunohistochemistry for a large number of human tissues, cancers and cell lines: The Human Protein Atlas.

Bioinformatics

• Gene Symbol: AIRE