Aurora C Overexpression Lysate (Native)

Images

 
Western Blot: Aurora C Overexpression Lysate (Adult Normal) [NBL1-07862] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Aurora C.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Aurora C Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC213890 and based on accession number NM_001015878. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens aurora kinase C (AURKC), transcript variant 1, mRNA.
Gene
AURKC

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
35.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBL1-07862 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25318984)

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Aurora C

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Aurora C Overexpression Lysate (Native)

  • AIE2AIK3
  • ARK3Serine/threonine-protein kinase aurora-C
  • AurC
  • aurora kinase CAurora/IPL1/Eg2 protein 2
  • Aurora/IPL1-related kinase 3
  • aurora-C
  • Aurora-related kinase 3
  • EC 2.7.11
  • EC 2.7.11.1
  • serine/threonine kinase 13 (aurora/IPL1-like)
  • serine/threonine-protein kinase 13
  • STK13ARK-3

Background

This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq]. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-294
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, MiAr
NBP1-51843
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP2-37471
Species: Hu
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready
NB500-171
Species: Hu, Mu, Rt, Xp, Ye
Applications: WB, ChIP, ICC/IF, IHC, IHC-P
H00027033-B02P
Species: Hu
Applications: WB, ELISA, ICC/IF
NB110-85519
Species: Hu, Mu
Applications: WB, ELISA, IHC, IP
NB100-294
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP, MiAr
H00009266-M02
Species: Hu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB100-547
Species: Hu
Applications: WB, ICC/IF, IP, PLA
NBP1-81765
Species: Hu
Applications: IHC, IHC-P
NBP1-69668
Species: Hu
Applications: WB
NBP2-07087
Species: Hu
NB100-56508
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC, IHC-P, CyTOF-ready, Flow-CS
NBP1-97587
Species: Hu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP2-12892
Species: Hu
Applications: WB, ICC/IF
NBL1-07862
Species: Hu
Applications: WB

Publications for Aurora C Lysate (NBL1-07862)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Reviews for Aurora C Lysate (NBL1-07862) (0)

There are no reviews for Aurora C Lysate (NBL1-07862). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Aurora C Lysate (NBL1-07862) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Aurora C Products

Bioinformatics Tool for Aurora C Lysate (NBL1-07862)

Discover related pathways, diseases and genes to Aurora C Lysate (NBL1-07862). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Aurora C Lysate (NBL1-07862)

Discover more about diseases related to Aurora C Lysate (NBL1-07862).
 

Pathways for Aurora C Lysate (NBL1-07862)

View related products by pathway.

PTMs for Aurora C Lysate (NBL1-07862)

Learn more about PTMs related to Aurora C Lysate (NBL1-07862).
 

Research Areas for Aurora C Lysate (NBL1-07862)

Find related products by research area.

Blogs on Aurora C

There are no specific blogs for Aurora C, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Aurora C Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol AURKC