Aurora A Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC201583 and based on accession number NM_198435. The protein contains a C-terminal DDK tag.
Homo sapiens aurora kinase A (AURKA), transcript variant 4, mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for Aurora A
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for Aurora A Overexpression Lysate (Native)
- ARK1EC 220.127.116.11
- Aurora A
- aurora kinase AhARK1
- aurora/IPL1-like kinase
- Aurora/IPL1-related kinase 1
- Aurora-related kinase 1
- Breast tumor-amplified kinase
- breast-tumor-amplified kinase
- EC 2.7.11
- IPL1-related kinase
- serine/threonine kinase 6
- serine/threonine protein kinase 15
- Serine/threonine-protein kinase 15
- serine/threonine-protein kinase 6
- Serine/threonine-protein kinase aurora-A
- STK15serine/threonine kinase 15
The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1-6 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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