Aurora A Antibody (1A14)

Western Blot: Aurora A Antibody (1A14) [NBP2-50041] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 h was probed with NBP2-50041. Nocodazole is a microtubule poymerization inhibitor which induces cells to halt at G2/M phase and induces Aurora A expression. NBP2-50041 binds strongly and cleanly to a band at about 46 kDa. Right: Blot of recombinant human Aurora A, B and C was probed with NBP2-50041.

Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1A14) [NBP2-50041] - HeLa cell cultures stained with NBP2-50041 (green). Aurora A localizes in spindle poles and mitotic spindles at late mitosis. Counterstained is chicken polyclonal antibody against Vimentin (NB300-223, red). Blue is a DNA stain.

Product Discontinued

Aurora A Antibody (1A14) Summary

• Immunogen: Recombinant full length human Aurora A. [UniProt# O14965]
• Specificity: This antibody does not cross-react with Aurora B or Aurora C.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AURKA
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:500 - 1:1000
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 50% Glycerol
• Preservative: 0.07% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Aurora A Antibody (1A14)

• AIK
• AIKAurA
• ARK1
• ARK-1
• ARK1EC 2.7.11.1
• AURA
• AURKA
• Aurora A
• aurora kinase AhARK1
• aurora/IPL1-like kinase
• Aurora/IPL1-related kinase 1
• Aurora-related kinase 1
• Breast tumor-amplified kinase
• breast-tumor-amplified kinase
• BTAK
• BTAKAURORA2
• EC 2.7.11
• IPL1-related kinase
• serine/threonine kinase 6
• serine/threonine protein kinase 15
• Serine/threonine-protein kinase 15
• serine/threonine-protein kinase 6
• Serine/threonine-protein kinase aurora-A
• STK15
• STK15serine/threonine kinase 15
• STK6
• STK6MGC34538
• STK7

Background

The protein encoded by the Aurora A gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Aurora A Antibody (NBP2-50041)(2)

Publications using NBP2-50041

• Wu T, Dong J, Fu J et al. The mechanism of acentrosomal spindle assembly in human oocytes Science (New York, N.Y.) 2022-11-18 [PMID: 36395215]
• So C, Seres K. B, et al. A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes. Science 2019-06-28 [PMID: 31249032]

Bioinformatics

• Gene Symbol: AURKA
• Uniprot:
  • O14965()
  • O14965()