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Aurora A Antibody (1A14)

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Western Blot: Aurora A Antibody (1A14) [NBP2-50041] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 h was probed with NBP2-50041. Nocodazole is a microtubule poymerization inhibitor which induces cells to halt at G2/M phase and induces Aurora A expression. NBP2-50041 binds strongly and cleanly to a band at about 46 kDa. Right: Blot of recombinant human Aurora A, B and C was probed with NBP2-50041.
Western Blot: Aurora A Antibody (1A14) [NBP2-50041] - Left: Analysis of HeLa cells treated with 100ng/ml nocodazole for 18 h was probed with NBP2-50041. Nocodazole is a microtubule poymerization inhibitor which induces ...read more
Immunocytochemistry/ Immunofluorescence: Aurora A Antibody (1A14) [NBP2-50041] - HeLa cell cultures stained with NBP2-50041 (green). Aurora A localizes in spindle poles and mitotic spindles at late mitosis. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clone
1A14
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Aurora A Antibody (1A14) Summary

Immunogen
Recombinant full length human Aurora A. [UniProt# O14965]
Specificity
This antibody does not cross-react with Aurora B or Aurora C.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
AURKA
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:1000
Theoretical MW
46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-50041.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
10mM Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Aurora A Antibody (1A14)

  • AIK
  • AIKAurA
  • ARK1
  • ARK-1
  • ARK1EC 2.7.11.1
  • AURA
  • AURKA
  • Aurora A
  • aurora kinase AhARK1
  • aurora/IPL1-like kinase
  • Aurora/IPL1-related kinase 1
  • Aurora-related kinase 1
  • Breast tumor-amplified kinase
  • breast-tumor-amplified kinase
  • BTAK
  • BTAKAURORA2
  • EC 2.7.11
  • IPL1-related kinase
  • serine/threonine kinase 6
  • serine/threonine protein kinase 15
  • Serine/threonine-protein kinase 15
  • serine/threonine-protein kinase 6
  • Serine/threonine-protein kinase aurora-A
  • STK15
  • STK15serine/threonine kinase 15
  • STK6
  • STK6MGC34538
  • STK7

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aurora A Antibody (NBP2-50041)(1)


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Bioinformatics

Gene Symbol AURKA
Uniprot
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