Attractin Antibody

Western blot shows human plasma. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human Attractin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7238) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Attractin at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Attractin was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Sheep Anti-Human Attractin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7238) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Attractin Antibody Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Attractin
  Ala84-Gln1272
  Accession # NP_647538
• Specificity: Detects human Attractin in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ATRN
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Attractin Antibody

• ATRN
• Attractin
• DPPT-L
• KIAA0548
• Mahogany Protein
• MGC126754
• MGCA

Background

Attractin (ATRN), also known as DPPT-L, is an approximately 200 kDa transmembrane glycoprotein that shows dipeptidyl peptidase activity similar to DPPIV/CD26. Attractin is involved in a variety of processes including monocyte-T cell adhesion, axon myelination, melanocyte pigment synthesis, and energy homeostasis. The extracellular region of human Attractin contains one EGF-like domain, one CUB domain, six Kelch repeats, four PSI domains, one C-type lectin domain, and two laminin EGF-like domains. Alternate splicing of human Attractin generates a secreted isoform that lacks the transmembrane and cyotplasmic regions. Attractin is transiently upregulated during T cell activation before expression switches to the 175 kDa secreted isoform which is released into the circulation. Soluble Attractin is preferentially expressed by leukocytes and differentiating neurons. It blocks neurite formation and is elevated in the CSF of astrocytoma patients. Within aa 84-1272, human Attractin shares 95% aa sequence identity with mouse and rat Attractin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Attractin Antibody (AF7238)(1)

Publication using AF7238

• Parashara, P;Gao, L;Riglos, A;Sidhu, SB;Lartey, D;Marks, T;Williams, C;Siauw, G;Ostrem, AIL;Siebold, C;Kinnebrew, M;Riffle, M;Gunn, TM;Kong, JH; The E3 ubiquitin ligase MGRN1 targets melanocortin receptors MC1R and MC4R via interactions with transmembrane adapters bioRxiv : the preprint server for biology 2025-03-26 [PMID: 40196599] (Western Blot, Human)

Bioinformatics

• Gene Symbol: ATRN
• Uniprot:
  • NP_647538()