ATPase Na+/K+ beta 3 Antibody (2F4) - Azide and BSA Free

ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05] - Detection limit for recombinant GST tagged ATP1B3 is approximately 3ng/ml as a capture antibody.

ELISA: ATPase Na+/K+ beta 3 Antibody (2F4) [H00000483-M05] - Detection limit for recombinant GST tagged ATP1B3 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu, Bv
• Applications: WB, ELISA, ICC/IF
• Clone: 2F4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATPase Na+/K+ beta 3 Antibody (2F4) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ATP1B3 (AAH11835, 1 a.a. ~ 279 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MTKNEKKSLNQSLAEWKLFIYNPTTGEFLGRTAKSWGLILLFYLVFYGFLAALFSFTMWVMLQTLNDEVPKYRDQIPSPGLMVFPKPVTALEYTFSRSDPTSYAGYIEDLKKFLKPYTLEEQKNLTVCPDGALFEQKGPVYVACQFPISLLQACSGMNDPDFGYSQGNPCILVKMNRIIGLKPEGVPRIDCVSKNEDIPNVAVYPHNGMIDLKYFPYYGKKLHVGYLQPLVAVQVSFAPNNTGKEVTVECKIDGSANLKSQDDRDKFLGRVMFKITARA
• Specificity: ATP1B3 - ATPase, Na+/K+ transporting, beta 3 polypeptide
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP1B3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control. Use in Western blot reported in scientific literature (PMID 28134274).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATPase Na+/K+ beta 3 Antibody (2F4) - Azide and BSA Free

• ATP1B3
• ATPase Na+/K+ beta 3
• ATPase, Na+/K+ transporting, beta 3 polypeptide
• ATPB-3
• CD antigen CD298
• CD298 antigen
• CD298
• FLJ29027
• Na, K-ATPase beta-3 polypeptide
• sodium/potassium-dependent ATPase beta-3 subunit
• Sodium/potassium-dependent ATPase subunit beta-3
• sodium/potassium-transporting ATPase beta-3 chain
• sodium/potassium-transporting ATPase subunit beta-3

Background

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATPase Na+/K+ beta 3 Antibody (H00000483-M05)(2)

Publications using H00000483-M05

• Tharkeshwar AK, Trekker J, Vermeire W et al. A novel approach to analyze lysosomal dysfunctions through subcellular proteomics and lipidomics: the case of NPC1 deficiency. Sci Rep. 2017-01-30 [PMID: 28134274] (WB)
• Goossens K, Vandaele L, Wydooghe E et al. The importance of adequate fixation for immunofluorescent staining of bovine embryos. Reprod Domest Anim. 2011-03-03 [PMID: 21366723]

Bioinformatics

• Gene Symbol: ATP1B3
• Entrez:
  • 483(Human)
• OMIM:
  • 601867(Human)
• Uniprot:
  • AAH11835(Human)