ATPase Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 488

ATPase Antibody [Alexa Fluor™ Plus 488] Summary

• Immunogen: Produced in rabbits immunized with E. coli-derived Human ATPase fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DNAH8
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Antigen and protein A Affinity-purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for ATPase Antibody [Alexa Fluor™ Plus 488]

• ATPase
• axonemal
• dynein, axonemal, heavy chain 8
• dynein, axonemal, heavy polypeptide 8
• FLJ25850
• FLJ36115
• FLJ36334
• hdhc9

Background

CATALYTIC ACTIVITY: ATP + H2O = ADP + phosphate. SUBCELLULAR LOCATION: Membrane; multi-pass membrane protein (By similarity). ALTERNATIVE PRODUCTS: 2 named isoforms produced by alternative splicing. SIMILARITY: Belongs to the cation transport ATPase (P-type) family. Type V subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for ATPase Antibody (NBP2-98188AFP488). (Showing 1 - 4 of 4 FAQ).

1. I have phosphate in my enzyme. What can I do?
   • You can dialyse or desalt the enzyme into a phosphate-free buffer. Alternatively, you can use a special resin (PiBind) to remove the phosphate.
2. I have 5% DMSO in my assay. Can I use PiColorLock Gold?
   • Yes, the reagent is designed for drug screening work and other situations that require DMSO.
3. I have a high background in my ATPase assay and I definitely do not have free phosphate in my sample
   • This is almost always due to inadequate mixing of the special stabilizer with the sample and detection reagent. Make sure the stabilizer is pipetted up and down several times to ensure thorough mixing.
4. I would like to measure the conversion of pyrophosphate to phosphate. Can I use the PiColorLock Gold Phosphate Detection System for this purpose?
   • Yes, only the phosphate will give a signal; pyrophosphate will not.

Bioinformatics

• Gene Symbol: DNAH8