ATP6V1G1 Overexpression Lysate (Native)

Images

 
Western Blot: ATP6V1G1 Overexpression Lysate (Adult Normal) [NBL1-07846] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ATP6V1G1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

ATP6V1G1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC203317 and based on accession number NM_004888. The protein contains a C-terminal DDK tag.
Epitope
C-terminal Myc/DDK
Specificity
Homo sapiens ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 (ATP6V1G1), mRNA.
Gene
ATP6V1G1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
13.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for ATP6V1G1 Overexpression Lysate (Native)

  • ATP6G1V-ATPase 13 kDa subunit 1
  • ATP6GATP6J
  • ATP6GL
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump), member J
  • ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G isoform 1
  • ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1
  • DKFZp547P234
  • vacuolar ATP synthase subunit M16
  • vacuolar H(+)-ATPase subunit G 1
  • Vacuolar proton pump subunit G 1
  • Vacuolar proton pump subunit M16
  • V-ATPase subunit G 1
  • Vma10
  • V-type proton ATPase subunit G 1

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for ATP6V1G1 Lysate (NBL1-07846) (0)

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Bioinformatics

Gene Symbol ATP6V1G1