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ATP6V1D Antibody

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Western Blot: ATP6V1D Antibody [NBP2-92709] - Analysis of extracts of various cell lines, using ATP6V1D at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 60s.
Western Blot: ATP6V1D Antibody [NBP2-92709] - Analysis of extracts of various cell lines, using ATP6V1D at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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ATP6V1D Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-247 of human ATP6V1D (NP_057078.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATP6V1D
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for ATP6V1D Antibody

  • ATP6M
  • ATPase, H+ transporting lysosomal, member M
  • ATPase, H+ transporting, lysosomal (vacuolar proton pump)
  • ATPase, H+ transporting, lysosomal 34kDa, V1 subunit D
  • H(+)-transporting two-sector ATPase, subunit M
  • vacuolar ATP synthase subunit D
  • vacuolar H-ATPase subunit D
  • vacuolar proton pump D subunit
  • vacuolar proton pump delta polypeptide
  • Vacuolar proton pump subunit D
  • vacuolar proton-ATPase subunit D
  • VATDV-ATPase D subunit
  • V-ATPase 28 kDa accessory protein
  • V-ATPase subunit D
  • VMA8
  • V-type proton ATPase subunit D

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunohistochemistry-Paraffin: p97/VCP Antibody (5) [NB120-11433] - Both normal and cancer biopsies of deparaffinized Human brain tissue tissues.
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Immunocytochemistry/Immunofluorescence: DIO2 Antibody [NBP1-00178] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton.Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Western Blot: TOMM34 Antibody (2A9) [NBP2-00892] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TOMM34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TOMM34.
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Western Blot: ATP6V0D1 Antibody (2G12) [H00009114-M01] - Analysis of ATP6V0D1 expression in transfected 293T cell line by ATP6V0D1 monoclonal antibody (M01), clone 2G12.Lane 1: ATP6V0D1 transfected lysate(40.3 KDa).Lane 2: Non-transfected lysate.
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Publications for ATP6V1D Antibody (NBP2-92709) (0)

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Bioinformatics

Gene Symbol ATP6V1D