ATP6V1B2 Antibody (M1) - Azide and BSA Free

ELISA: ATP6V1B2 Antibody (M1) [H00000526-M02] - Detection limit for recombinant GST tagged ATP6V1B2 is approximately 1ng/ml as a capture antibody.

Sandwich ELISA: ATP6V1B2 Antibody (M1) [H00000526-M02] - Detection limit for recombinant GST tagged ATP6V1B2 is approximately 0.03ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA
• Clone: M1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ATP6V1B2 Antibody (M1) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ATP6V1B2 (AAH03100, 1 a.a. ~ 511 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MALRAMRGIVNGAAPELPVPTGGPAVGAREQALAVSRNYLSQPRLTYKTVSGVNGPLVILDHVKFPRYAEIVHLTLPDGTKRSGQVLEVSGSKAVVQVFEGTSGIDAKKTSCEFTGDILRTPVSEDMLGRVFNGSGKPIDRGPVVLAEDFLDIMGQPINPQCRIYPEEMIQTGISAIDGMNSIARGQKIPIFSAAGLPHNEIAAQICRQAGLVKKSKDVVDYSEENFAIVFAAMGVNMETARFFKSDFEENGSMDNVCLFLNLANDPTIERIITPRLALTTAEFLAYQCEKHVLVILTDMSSYAEALREVSAAREEVPGRRGFPGYMYTDLATIYERAGRVEGRNGSITQIPILTMPNDDITHPIPDLTGYITEGQIYVDRQLHNRQIYPPINVLPSLSRLMKSAIGEGMTRKDHADVSNQLYACYAIGKDVQAMKAVVGEEALTSDDLLYLEFLQKFERNFIAQGPYENRTVFETLDIGWQLLRIFPKEMLKRIPQSTLSEFYPRDSAKH
• Specificity: ATP6V1B2 - ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ATP6V1B2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against recombinant protein on ELISA. GST alone used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for ATP6V1B2 Antibody (M1) - Azide and BSA Free

• 000 subunit
• 56/58kD, isoform 2
• ATP6B2ATPase, H+ transporting, lysosomal (vacuolar proton pump), beta polypeptide
• ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
• Endomembrane proton pump 58 kDa subunit
• H+ transporting two-sector ATPase
• HO57ATP6B1B2
• vacuolar H+-ATPase 56
• Vacuolar proton pump subunit B 2
• VATB
• V-ATPase B2 subunit
• V-ATPase subunit B 2
• Vma2
• VPP3ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B, isoform 2
• V-type proton ATPase subunit B, brain isoform

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of two V1 domain B subunit isoforms and is the only B isoform highly expressed in osteoclasts.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ATP6V1B2 Antibody (H00000526-M02)(2)

Publications using H00000526-M02

• Eriksson I, Joosten M, Roberg K, Ollinger K. The histone deacetylase inhibitor trichostatin A reduces lysosomal pH and enhances cisplatin-induced apoptosis. Exp Cell Res. 2012-10-12 [PMID: 23063877]
• Nilsson C, Roberg K, Grafstrom RC, Ollinger K. Intrinsic differences in cisplatin sensitivity of head and neck cancer cell lines: Correlation to lysosomal pH. Head Neck;32(9):1185-94. 2010-09-01 [PMID: 20029982]

Bioinformatics

• Gene Symbol: ATP6V1B2
• Entrez:
  • 526(Human)
• OMIM:
  • 606939(Human)
• Uniprot:
  • AAH03100(Human)