ATP6V0D1 Antibody

Western Blot: ATP6V0D1 Antibody [NBP2-15517] - Sample (30 ug of whole cell lysate) A: IMR32 10% SDS PAGE gel, diluted at 1:500.

Immunohistochemistry: ATP6V0D1 Antibody [NBP2-15517] - Immunohistochemical analysis of agarose-embedded zebrafish embryo, using ATP6V0D1 antibody (NBP2-15517) at 1:200 dilution. (This image was provided courtesy of the Schilling Lab at UC, Irvine.)

• Reactivity: Hu, Ze, Mu, Rt, Bv, Ch, Xp, Ze
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

ATP6V0D1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP6V0D1. The exact sequence is proprietary.
• Predicted Species: Mouse (99%), Rat (99%), Chicken (98%), Xenopus (95%), Zebrafish (94%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP6V0D1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:3000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (95%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol, 1% BSA
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ATP6V0D1 Antibody

• 32 kDa accessory protein
• ATP6D
• ATP6DV
• ATP6V0
• ATPase, H+ transporting, lysosomal (vacuolar proton pump), member D
• ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d isoform 1
• ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
• FLJ43534
• H(+)-transporting two-sector ATPase, subunit D
• P39
• Vacuolar proton pump subunit d 1
• V-ATPase 40 kDa accessory protein
• V-ATPase AC39 subunit
• V-ATPase, subunit D
• VATX
• Vma6
• VPATPDV-ATPase subunit d 1
• V-type proton ATPase subunit d 1

Background

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ATP6V0D1
• Uniprot:
  • P61421(Human)