ATP6V0A4 Antibody

Western Blot: ATP6V0A4 Antibody [NBP1-32904] - Sample (30 ug of whole cell lysate) A: A431 B: Molt-4 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: ATP6V0A4 Antibody [NBP1-32904] - Sample: A431 cells were fixed in iced-cold MeOH for 5 min. Green: ATP6V0A4 protein stained by ATP6V0A4 antibody diluted at 1:500. Blue: Hoechst 33342 staining.

Product Discontinued

ATP6V0A4 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human ATP6V0A4. The exact sequence is proprietary.
• Localization: Apical cell membrane, Multi-pass membrane protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATP6V0A4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 96 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse (89%), Bovine (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for ATP6V0A4 Antibody

• A4
• ATP6N2
• ATP6V0
• ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B
• ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38kD)
• ATPase, H+ transporting, lysosomal V0 subunit a4
• MGC130016
• MGC130017
• noncatalytic accessory protein 1B
• RDRTA2
• RTA1C
• RTADR
• STV1
• vacuolar proton pump 116 kDa accessory subunit
• vacuolar proton pump, subunit 2
• vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform
• V-ATPase 116 kDa
• VPH1
• VPP2
• V-type proton ATPase 116 kDa subunit a isoform 4
• V-type proton ATPase 116 kDa subunit a

Background

The ATP6V0A4 gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sor

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ATP6V0A4