ATF6 Antibody

Images

 
Western Blot: ATF6 Antibody [NBP1-77251] - Analysis in MCF7 cell lysate with antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: ATF6 Antibody [NBP1-77251] - A20 with ATF6 antibody at 10 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

ATF6 Antibody Summary

Immunogen
Antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of human ATF6. The immunogen is located within amino acids 30 - 80 of ATF6.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATF6
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.5 - 1 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
Application Notes
ATF6 antibody can be used for the detection of ATF6 by Western blot at 0.5 - 1 mg/ml.
Control Peptide
ATF6 Protein (NBP1-77251PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATF6 Antibody

  • Activating transcription factor 6 alpha
  • activating transcription factor 6
  • atf6 a
  • ATF6A
  • ATF6-alpha
  • cAMP-dependent transcription factor ATF-6 alpha
  • cyclic AMP-dependent transcription factor ATF-6 alpha

Background

Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR. ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes. ATF6 has two isoforms (ATF6alpha and ATF6beta); only ATF6alpha is recognized by this antibody.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ATF6 Antibody (NBP1-77251). (Showing 1 - 2 of 2 FAQ).

  1. We are looking for an antibody specific to ATF6 alpha that will not cross-react with ATF6 beta. Would you please help confirm if any of the following are ATF6 alpha specific antibodies: NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439?
    • The antibodies NBP1-77251 and NBP1-76675 are specific to ATF6 alpha and should not cross-react with ATF6 beta.
  2. May we ask if ATF6 is ATF6 alpha? We are looking for ATF6 alpha antibody for our customer, but found none from Novus. Only ATF6 antibodies. However, after checking http://www.uniprot.org/uniprot/P18850, I found ATF6 and ATF6 alpha seem identical. Would you please help confirm if NBP1-40256, NBP1-77251, NBP1-76675 & NBP1-41439 are all ATF6 alpha antibodies?
    • The antibodies NBP1-77251, NBP1-76675 are specific to ATF6A and should not cross-react with ATF6B.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ATF6 Antibody (NBP1-77251)

Discover related pathways, diseases and genes to ATF6 Antibody (NBP1-77251). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for ATF6 Antibody (NBP1-77251)

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Blogs on ATF6.

ATF6 - monitoring and regulating protein folding under cellular stress
During times of cellular stress overloading of the protein folding machinery leads to the accumulation of incorrectly folded proteins. This triggers the unfolded protein response (UPR) in order to try to reestablish homeostasis or, if this fails, t...  Read full blog post.

ATF6 - a key target in alcohol-induced fatty liver disease?
Untitled DocumentThe ATF6 endoplasmic reticulum (ER) stress-regulated transcription factor is constitutively expressed and plays a central role in the mammalian unfolded protein response (UPR). This fundamental pathway is responsibl...  Read full blog post.

A Key to Fight Stress: ATF6
The protein ATF6 is a constitutively expressed transcription factor that is a key mediator of the unfolded protein response (UPR) that allows mammalian cells to maintain cellular homeostasis under conditions of environmental and physiological stress....  Read full blog post.

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Bioinformatics

Gene Symbol ATF6
Uniprot