ATF2 [p Thr52] Antibody - Azide Free

Western Blot: ATF2 [p Thr52] Antibody [NBP2-29528] - Western blot of UACC903 melanoma cell line lysate (DMSO) showing specific immunolabeling of the ~72k ATF2 protein phosphorylated at Thr52. Immunolabeling is reduced by treatment of the lysate with the PKC catalytic inhibitor Go6850. Image courtesy of Eric Lau and Ze'ev Ronai.

• Reactivity: Hu, Mu, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

ATF2 [p Thr52] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit ATF2 [p Thr52] Antibody - Azide Free (NBP2-29528) is a polyclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding THr52 conjugated to KLH. Accession # P15336
• Modification: p Thr52
• Specificity: Specific for endogenous levels of the ~72 kDa ATF2 protein phosphorylated at Thr52. Immunolabeling of the ATF2 band is reduced by treatment of the cells with the PKC catalytic inhibitor Go6850.
• Preparation Method: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and dephosphopeptide affinity columns.
• Predicted Species: Primate (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ATF2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:250
• Theoretical MW: 74 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Non-human primate. The antibody has been directly tested for reactivity in Western blots with UACC903 melanoma cells.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for ATF2 [p Thr52] Antibody - Azide Free

• activating transcription factor 2formerly
• ATF2
• cAMP responsive element binding protein 2
• cAMP-responsive element-binding protein 2
• CREB2
• CREBP1
• CRE-BP1
• cyclic AMP-dependent transcription factor ATF-2
• HB16
• MGC111558
• mXBP
• TREB7

Background

The transcription factor ATF2 is a member of the ATF/CREB family of leucine zipper proteins. In response to stress stimuli, it activates a variety of gene targets that are involved in oncogenesis, and has been correlated with maintenance of a cancer cell phenotype (Vlahopoulos et al., 2008). Inhibiting ATF2 impedes melanoma development and elicits tumor suppressor function (Bhoumik et al., 2008). To act as a tumor suppressor, ATF2 must localize at the mitochondria, and phosphorylation at Thr52 by PKC-epsilon regulates this translocation (Lau et al., 2012).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ATF2
• Entrez:
  • 1386(Human)
• Uniprot:
  • P15336()