Ovastacin Antibody [Unconjugated]

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and OVCAR-3 human ovarian carcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human Ovastacin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8549) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Ovastacin at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of K562 human chronic myelogenous leukemia cell line and OVCAR‑3 human ovarian carcinoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Ovastacin at approximately 69 kDa (as indicated) using 1:100 dilution of Rabbit Anti-Human Ovastacin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8549) . This experiment was conducted under reducing conditions and using the12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

• Reactivity: Hu
• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Ovastacin Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Ovastacin
  Ala24-Asp431
  Accession # Q6HA08
• Specificity: Detects human Ovasticin in direct ELISAs and Western blots. In direct ELISAs, less then 1% cross-reactivity with recombinant human BMP-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASTL
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 1:100 dilution
  • Western Blot 1:1000 dilution

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
• Buffer: Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ovastacin Antibody [Unconjugated]

• astacin-like metallo-endopeptidase (M12 family)
• Astacin-Like Metalloendopeptidase
• ASTL
• Oocyte Astacin
• Ovastacin

Background

Ovastacin (ASTL, astacin-like metallo-endopeptidase and Oocyte astacin) is a 50 kDa  member of the peptidase M12A family of proteins. Ovastacin is an oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization, it plays a role in the polyspermy inhibition. Human Ovastacin is a metalloendopeptidase that contains three zinc binding domains. Ovastacin cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding. Over aa 24-431, human Ovastacin shares 68% aa identity with mouse Ovastacin.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASTL
• Uniprot:
  • Q6HA08()