Aspartate Aminotransferase Antibody

Images

 
Western Blot: Aspartate Aminotransferase Antibody [NBP2-57518] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma, human liver tissue and human tonsil tissue.
Immunocytochemistry/ Immunofluorescence: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining in human heart muscle and lymph node tissues using anti-GOT1 antibody. Corresponding GOT1 RNA-seq ...read more
Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining of human heart muscle shows high expression.
Immunohistochemistry-Paraffin: Aspartate Aminotransferase Antibody [NBP2-57518] - Staining of human lymph node shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Aspartate Aminotransferase Antibody Summary

Immunogen
This antibody was developed against a recombinant protein corresponding to the following amino acid sequence: IGMFSFTGLNPKQVEYLVNEKHIYLLPSGRINVSGLTTKNLDYVATSIHEAVTKIQ
Specificity
Specificity of human Aspartate Aminotransferase antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Predicted Species
Mouse (96%), Rat (91%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GOT1
Purity
Affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control
Aspartate Aminotransferase Lysate (NBP2-66128)
Control Peptide
Aspartate Aminotransferase Recombinant Protein Antigen (NBP2-57518PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for Aspartate Aminotransferase Antibody

  • aspartate aminotransferase, cytoplasmic
  • EC 2.6.1.1
  • GIG18
  • Glutamate oxaloacetate transaminase 1
  • glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1)
  • growth-inhibiting protein 18
  • Transaminase A

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Diseases for Aspartate Aminotransferase Antibody (NBP2-57518)

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Bioinformatics

Gene Symbol GOT1