Asparagine synthetase Antibody

Western Blot: Asparagine synthetase Antibody [H00000440-D01P] - Analysis of ASNS expression in transfected 293T cell line by ASNS polyclonal antibody.Lane 1: ASNS transfected lysate(64.40 KDa).Lane 2: Non-transfected lysate.

Product Discontinued

Asparagine synthetase Antibody Summary

• Description: Quality control test: Antibody reactive against mammalian transfected lysate.
• Immunogen: ASNS (NP_001664.2, 1 a.a. - 561 a.a.) full-length human protein. MCGIWALFGSDDCLSVQCLSAMKIAHRGPDAFRFENVNGYTNCCFGFHRLAVVDPLFGMQPIRVKKYPYLWLCYNGEIYNHKKMQQHFEFEYQTKVDGEIILHLYDKGGIEQTICMLDGVFAFVLLDTANKKVFLGRDTYGVRPLFKAMTEDGFLAVCSEAKGLVTLKHSATPFLKVEPFLPGHYEVLDLKPNGKVASVEMVKYHHCRDEPLHALYDNVEKLFPGFEIETVKNNLRILFNNAVKKRLMTDRRIGCLLSGGLDSSLVAATLLKQLKEAQVQYPLQTFAIGMEDSPDLLAARKVADHIGSEHYEVLFNSEEGIQALDEVIFSLETYDITTVRASVGMYLISKYIRKNTDSVVIFSGEGSDELTQGYIYFHKAPSPEKAEEESERLLRELYLFDVLRADRTTAAHGLELRVPFLDHRFSSYYLSLPPEMRIPKNGIEKHLLRETFEDSNLIPKEILWRPKEAFSDGITSVKNSWFKILQEYVEHQVDDAMMANAAQKFPFNTPKTKEGYYYRQVFERHYPGRADWLSHYWMPKWINATDPSARTLTHYKSAVKA
• Specificity: ASNS - asparagine synthetase,
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASNS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB and also on transfected lysate in WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Asparagine synthetase Antibody

• ASNS
• ASNSD
• asparagine synthetase (glutamine-hydrolyzing)
• asparagine synthetase [glutamine-hydrolyzing]
• asparagine synthetase
• Cell cycle control protein TS11
• EC 6.3.5.4
• Glutamine Dependent Asparagine Synthetase
• Glutamine-dependent asparagine synthetase
• TS11 Cell Cycle Control Protein
• TS11

Background

The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. There are three alternatively spliced transcript variants encoding the same protein described for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for Asparagine synthetase Antibody (H00000440-D01P). (Showing 1 - 1 of 1 FAQ).

1. A while back I ordered around 18 antibodies and have been in the process of testing them. And I realized that a couple of them under the application section on the website said: Antibody reactive against Recombinant Protein with GST tag on ELISA and Western Blot and also on transfected lysate in western blot. GST tag alone is used as a negative control. I was wondering if that means the antibody won't detect the endogenous epitope and will only detect the recombinant protein that's been transfected? Or if it can be used also to detect the endogenously expressed protein. So the two antibodies in question are the H00050810-D01P Hdgfrp3 antibody and the H00000440-D01P Asparagine synthetase antibody. Both show a transfected lysate as a positive control for the antibodies. Are you aware of any positive control cell lines for detecting endogenous (non-transfected) protein for either or both of these antibodies?
   • The antibodies you described should be able to detect the endogenous protein, and we guarantee them for this purpose. We do our best to provide as much information as possible about the testing conditions of our antibodies, which is why we included the stated information. I do not know of any non-transfected cell lines that are suitable as specific positive controls for either asparagine synthetase or Hdgfrp3. K563 cells and muscle tissue express asparagine synthetase, but this could be influenced by treatment of the cells. MCF-7 cells may be a good positive control for Hdgfrp3.

Bioinformatics

• Gene Symbol: ASNS
• Entrez:
  • 440(Human)
• Uniprot:
  • NP_001664.2(Human)