Asparagine synthetase Antibody (SC67-07)

Western Blot: Asparagine synthetase Antibody (SC67-07) [NBP2-67593] - Western blot analysis of Asparagine synthetase on human lung using anti-Asparagine synthetase antibody at 1/500 dilution.

Immunocytochemistry/Immunofluorescence: Asparagine synthetase Antibody (SC67-07) [NBP2-67593] - Staining Asparagine synthetase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Asparagine synthetase Antibody (SC67-07) [NBP2-67593] - Analysis of paraffin-embedded mouse stomach tissue using anti-Asparagine synthetase antibody. Counter stained with hematoxylin.

Western Blot: Asparagine synthetase Antibody (SC67-07) [NBP2-67593] - Analysis of Asparagine synthetase on different lysates using anti-Asparagine synthetase antibody at 1/1,000 dilution. Positive control: Lane 1: K562 Lane 2: Human skeletal muscle

Immunocytochemistry/Immunofluorescence: Asparagine synthetase Antibody (SC67-07) [NBP2-67593] - Staining Asparagine synthetase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Asparagine synthetase Antibody (SC67-07) [NBP2-67593] - Staining Asparagine synthetase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Asparagine synthetase Antibody (SC67-07) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Asparagine synthetase aa 502-538 / 561. (SwissProt: P08243 Human; SwissProt: Q61024 Mouse; SwissProt: P49088 Rat)
• Localization: Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ASNS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000-1:5000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Asparagine synthetase Antibody (SC67-07)

• ASNS
• ASNSD
• asparagine synthetase (glutamine-hydrolyzing)
• asparagine synthetase [glutamine-hydrolyzing]
• asparagine synthetase
• Cell cycle control protein TS11
• EC 6.3.5.4
• Glutamine Dependent Asparagine Synthetase
• Glutamine-dependent asparagine synthetase
• TS11 Cell Cycle Control Protein
• TS11

Background

Asparagine synthetase (AS) is a housekeeping enzyme responsible for the production of asparagine from aspartate and glutamine. Most mammalian cells express AS and regulate the level of activity in response to the concentration of asparagine in the medium. Certain tumor cells in contrast, exhibit little or no AS activity and rely on the surrounding medium as a source of exogenous asparagines (1). Therefore tumor cells may be selectively killed by a chemotherapeutic drug using asparaginase. This approach has been exploited in the treatment of certain cancers like childhood acute lymphoblastic leukemia (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASNS