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Asialoglycoprotein Receptor 2 Antibody (2327C) [Unconjugated]

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HEK293 human embryonic kidney cell line transfected with (A) human ASGR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) followed by APC-conjugated Anti-Rabbit IgG Secondary Antibody(Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
HEK293 human embryonic kidney cell line transfected with (A) human ASGR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) followed by ...read more
ASGR2 was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) at 1 µg/mL for 1 hour at room temperature followed by ...read more
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human ASGR2 Monoclonal Antibody (Catalog # MAB9970) followed by HRP-conjugated Anti-Rabbit IgG Secondary ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC
Clone
2327C
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB9970U-100UG
Alexa Fluor 405
FAB9970V-100UG
Alexa Fluor 488
FAB9970G-100UG
Alexa Fluor 594
FAB9970T-100UG
Alexa Fluor 647
FAB9970R-100UG
Alexa Fluor 700
FAB9970N-100UG
Alexa Fluor 750
FAB9970S-100UG
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB9970-SP
Carrier Free
FAB9970G-100UG
Carrier Free
FAB9970U-100UG
Carrier Free
FAB9970V-100UG
Carrier Free
FAB9970T-100UG
Carrier Free
FAB9970R-100UG
Carrier Free
FAB9970S-100UG
Carrier Free
FAB9970N-100UG
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Asialoglycoprotein Receptor 2 Antibody (2327C) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human ASGR2
Gln80-Ala311
Accession # P07307
Specificity
Detects human ASGR2 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 1-25 ug/mL
Reviewed Applications
Read 1 Review rated 5
using
MAB9970 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Asialoglycoprotein Receptor 2 Antibody (2327C) [Unconjugated]

  • ASGPR 2
  • ASGP-R 2
  • ASGPR2
  • ASGP-R2
  • ASGR2
  • asialoglycoprotein receptor 2
  • CLEC4H2
  • CLEC4H2FLJ60040
  • C-type lectin domain family 4 member H2
  • HBxAg-binding protein
  • HBXBP
  • Hepatic lectin H2
  • HL-2

Background

Asialoglycoprotein receptor 2 (ASGR2) is a subunit of the Asialoglycoprotein receptor. The asialoglycoprotein receptor is a hetero-oligomeric protein composed of major and minor subunits, which are encoded by different genes. The protein encoded by this gene is the less abundant minor subunit. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery. Expressed on hepatocytes, the Asialoglycoprotein receptor mediates endocytosis and lysosomal degradation of glycoproteins to mediate serum glycoprotein homeostasis. The ASGR receptor binds plasma glycoproteins which have had the terminal sialic acid residue removed. An alternatively spliced variant, H2, which can be shed, has been proposed as a marker for liver fibrosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Asialoglycoprotein Receptor 2 Antibody (MAB9970) (0)

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Review for Asialoglycoprotein Receptor 2 Antibody (MAB9970) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB9970:
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Flow Cytometry Asialoglycoprotein Receptor 2 MAB9970
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reviewed by:
Anonymous
 Flow Human 10/10/2019
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Summary

ApplicationFlow Cytometry
Sample TestedASGR over-expressing cells
SpeciesHuman
LotCLQI011909A

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB9970U
Alexa Fluor 405 FAB9970V
Alexa Fluor 488 FAB9970G
Alexa Fluor 594 FAB9970T
Alexa Fluor 647 FAB9970R
Alexa Fluor 700 FAB9970N
Alexa Fluor 750 FAB9970S
Learn about Custom Antibody Labeling

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Flow Cytometry Asialoglycoprotein Receptor 2 MAB9970
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 Anonymous
10/10/2019
Application: Flow
Species: Human
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