ASB5 Antibody - BSA Free

Western Blot: ASB5 Antibody [NBP1-32603] - Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ASB5 antibody diluted at 1:3000.

Immunocytochemistry/Immunofluorescence: ASB5 Antibody [NBP1-32603] - Analysis of methanol-fixed Hep G2, using antibody at 1:500 dilution.

Western Blot: ASB5 Antibody [NBP1-32603] - (30 ug whole cell lysate) A:A431 B:HeLa S3 C:Hep G2 10% SDS PAGE diluted at 1:1000

• Reactivity: Hu, Mu, Bv, Rb
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ASB5 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ASB5 Antibody - BSA Free (NBP1-32603) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ASB5. The exact sequence is proprietary.
• Predicted Species: Bovine (91%), Rabbit (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ASB5
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (83%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for ASB5 Antibody - BSA Free

• ankyrin repeat and SOCS box containing 5
• ankyrin repeat and SOCS box protein 5
• ankyrin repeat and SOCS box-containing 5
• ASB-5
• SOCS box protein ASB-5

Background

The protein encoded by this gene is a member of the ankyrin repeat and SOCS box-containing (ASB) family of proteins. They contain ankyrin repeat sequence and SOCS box domain. The SOCS box serves to couple suppressor of cytokine signalling (SOCS) proteins and their binding partners with the elongin B and C complex, possibly targeting them for degradation. Multiple alternatively spliced transcript variants have been described for this gene but their full length sequences are not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: ASB5