ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [Unconjugated]

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human ASAH2/N‑acylsphingosine Amidohydrolase-2
  Thr15-Ile761
  Accession # AAF86240
• Specificity: Detects human ASAH2/N‑acylsphingosine Amidohydrolase-2 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: ASAH2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ASAH2/N-acylsphingosine Amidohydrolase-2 Antibody [Unconjugated]

• ASAH2 N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
• ASAH2
• BCDase
• HNAC1
• LCDase
• Nacylsphingosine Amidohydrolase2
• NCDase
• N-CDase

Background

The human ASAH2 gene encodes N-acylsphingosine amidohydrolase-2, also known as neutral or non-lysosomal ceramidase. ASAH2 is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein (1). The enzyme is abundant in the brush border membranes of the intestine, but is also expressed in tissues such as kidney, brain and liver (2, 3). An N-terminally truncated form of human ASAH2 has been shown to localize to mitochondria (4). A major physiological function of ASAH2 is the metabolism of dietary sphingolipids, but the enzyme may also be involved in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate (3).

1. Tani, M. et al. (2003) J. Biol. Chem. 278:10523.
2. Kono, M. et al. (2006) J. Biol. Chem. 281:7324.
3. Mitsutake, S. et al. (2001) J. Biol. Chem. 276:26249.
4. El Bawab, S. et al. (2000) J. Biol. Chem. 275:21508.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ASAH2
• Uniprot:
  • AAF86240()