ARPC5 Antibody (SR34-02)

Western Blot: ARPC5 Antibody (SR34-02) [NBP2-67350] - Analysis of p16 ARC on different cell lysates using anti-p16 ARC antibody at 1/1,000 dilution. Positive control: Lane 1: MCF-7 Lane 2: SK-BR-3

Immunocytochemistry/Immunofluorescence: ARPC5 Antibody (SR34-02) [NBP2-67350] - Staining p16 ARC in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: ARPC5 Antibody (SR34-02) [NBP2-67350] - Immunohistochemical analysis of paraffin-embedded Human placenta tissue using anti-ARPC5 antibody. Counter stained with hematoxylin.

Flow Cytometry: ARPC5 Antibody (SR34-02) [NBP2-67350] - Analysis of SH-SY-5Y cells with p16 ARC antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody.

Immunohistochemistry-Paraffin: ARPC5 Antibody (SR34-02) [NBP2-67350] - Analysis of paraffin-embedded human spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ARPC5 Antibody (SR34-02) [NBP2-67350] - Analysis of paraffin-embedded mouse lung tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: ARPC5 Antibody (SR34-02) [NBP2-67350] - Analysis of paraffin-embedded mouse spleen tissue using anti-p16 ARC antibody. Counter stained with hematoxylin.

Product Discontinued

ARPC5 Antibody (SR34-02) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human ARPC5 aa 1-50 / 151. (SwissProt: O15511 Human; SwissProt: Q9CPW4 Mouse; SwissProt: Q4KLF8 Rat)
• Localization: Cytoplasm, Cytoskeleton, Nucleus, Cell projection.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ARPC5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for ARPC5 Antibody (SR34-02)

• actin related protein 2/3 complex, subunit 5, 16kDa
• ARC16subunit 5 (16 kD)
• MGC88523

Background

The ARPC5 gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has beenimplicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role ofthe protein encoded by th

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARPC5 Antibody (NBP2-67350)(1)

Publication using NBP2-67350

• Sindram, E;Caballero-Oteyza, A;Kogata, N;Huang, S;Alizadeh, Z;Gamez-Díaz, L;Fazlollahi, MR;Peng, X;Grimbacher, B;Way, M;Proietti, M; ARPC5 deficiency leads to severe early onset systemic inflammation and mortality Disease models & mechanisms 2023-06-29 [PMID: 37382373] (Western Blot)

Bioinformatics

• Gene Symbol: ARPC5