ARPC4 Antibody

Western Blot: ARPC4 Antibody [NBP1-06980] - Analysis of ARPC4 in Human Lymph Node lysate (35ug protein in RIPA buffer) using this antibody at 0.03 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARPC4 Antibody Summary

• Immunogen: Peptide with sequence C-ERHNKPEVEVR corresponding to internal region according to NP_005709.1.
• Epitope: internal region
• Specificity: This antibody is expected to recognise isoform a ( NP_005709.1).
• Predicted Species: Mouse (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARPC4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 19 kDa band observed in human lymph node lysates (calculated MW of 19.7 kDa band according to NP_005709.1).
• Control:

  ARPC4 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARPC4 Antibody

• actin related protein 2/3 complex, subunit 4, 20kDa
• actin-related protein 2/3 complex subunit 4
• arp2/3 protein complex subunit p20
• MGC13544
• p20-Arc
• subunit 4 (20 kD)

Background

This gene encodes one of seven subunits of the human Arp2/3 protein complex. The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells and has been conserved through evolution. The exact role of the protein encoded by this gene, the p20 subunit, has yet to be determined. Three transcript variants encoding two distinct isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARPC4 Antibody (NBP1-06980)(1)

Publication using NBP1-06980

• Rosentreter A, Hofmann A, Xavier CP et al. Coronin 3 involvement in F-actin-dependent processes at the cell cortex. Exp Cell Res 2007-03-10 [PMID: 17274980]

Bioinformatics

• Gene Symbol: ARPC4
• Entrez:
  • 10093(Human)
  • 68089(Mouse)
• Uniprot:
  • NP_005709.1(Human)