ARPC1A Antibody

Western Blot: ARPC1A Antibody [NBP2-11579] - Staining of fetal Mouse Brain (A) and adult Rat Brain (B) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ARPC1A Antibody [NBP2-11579] - Staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: ARPC1A Antibody [NBP2-11579] (0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ARPC1A Antibody Summary

• Description: Novus Biologicals Goat ARPC1A Antibody (NBP2-11579) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ARPC1A Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DFKTLESSIQGLR corresponding to C-Terminus according to NP_001177925.1.
• Epitope: CDFKTLESSIQGLR
• Specificity: This antibody is expected to recognize both reported isoforms (NP_006400.2; NP_001177925.1). No cross-reactiviry expected with ARPC1B.
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ARPC1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in HeLa lysates (calculated MW of 41.6 kDa band according to NP_006400.2). Approx. 37 kDa band observed in fetal mouse brain and adult rat brain lysates (calculated MW of 41.6 kDa band according to mouse NP_062741.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ARPC1A Antibody

• actin binding protein (Schizosaccharomyces pombe sop2-like)
• actin related protein 2/3 complex, subunit 1A (41 kD)
• actin related protein 2/3 complex, subunit 1A, 41kDa
• actin-related protein 2/3 complex subunit 1A
• SOP2Hs
• SOP2LArc40
• SOP2-like protein

Background

The ARPC1A gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they bo

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ARPC1A Antibody (NBP2-11579)(1)

Publication using NBP2-11579

• Laurila E, Savinainen K, Kuuselo R et al. Characterization of the 7q21-q22 amplicon identifies ARPC1A, a subunit of the Arp2/3 complex, as a regulator of cell migration and invasion in pancreatic cancer. Genes Chromosomes Cancer 2009-04-01 [PMID: 19145645]

Bioinformatics

• Gene Symbol: ARPC1A
• Entrez:
  • 10552(Human)
  • 56443(Mouse)
  • 81824(Rat)