ARID1A Antibody (3611) - Azide and BSA Free

Western Blot: ARID1A Antibody (3611) [NBP2-43566] - Analysis of whole cell extracts (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ARID1A antibody [3611] at a dilution of 1:500.

Immunocytochemistry/Immunofluorescence: ARID1A Antibody (3611) [NBP2-43566] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ARID1A stained by ARID1A antibody [3611] diluted at 1:200. Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody diluted at 1:500.

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566] - Mouse colon. ARID1A stained by ARID1A antibody [3611] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566] - Mouse intestine. ARID1A stained by ARID1A antibody [3611] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: ARID1A Antibody (3611) [NBP2-43566] - Human lung cancer. ARID1A stained by ARID1A antibody [3611] diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clone: 3611
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

ARID1A Antibody (3611) - Azide and BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human ARID1A. The exact sequence is proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ARID1A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 242 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rat (81%), Bovine (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for ARID1A Antibody (3611) - Azide and BSA Free

• ARID domain-containing protein 1A
• AT rich interactive domain 1A (SWI- like)
• AT rich interactive domain 1A (SWI-like)
• AT-rich interactive domain-containing protein 1A
• B120SWI-like protein
• BAF250a
• BAF250SWI/SNF complex protein p270
• BM029
• brain protein 120
• BRG1-associated factor 250
• BRG1-associated factor 250a
• C10rf4
• C1orf4SWI/SNF-related, matrix-associated, actin-dependent regulator of chromatinsubfamily F member 1
• chromatin remodeling factor p250
• hELD
• hOSA1
• matrix associated, actin dependent regulator of chromatin
• Osa homolog 1
• OSA1 nuclear protein
• OSA1
• P270
• SMARCF1
• subfamily f, member 1

Background

ARID1A encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain ARID1A genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARID1A