ARHGAP22 [p Ser397] Antibody

Images

 
Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to ...read more
Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Western Blot: ARHGAP22 [Phospo Ser22] Antibody [NBP1-44072] - Protein Molecular weight: 68 kDa Cell extracts from NIH 3T3cells that were transfected with either ...read more
Western Blot: ARHGAP22 [p Ser397] Antibody [NBP1-44073] - Lane 1: NIH3T3 cells transfected with a null vector. Lane 2: NIH3T3 cells transfected with ARHGAP22. Lane 3: NIH3T3 cells transfected with ARHGAP22 S22 to ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.07 mg/ml

Order Details

ARHGAP22 [p Ser397] Antibody Summary

Immunogen
A synthetic peptide corresponding to the region surrounding pS397.
Modification
p Ser397
Specificity
The NBP1-44073 antibody is specific for phosphorylated ARHGAP22 at Serine 397. It also recognizes the S22->A mutation but not the S397->A mutation.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ARHGAP22
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:10 - 1:500
Publications
Read Publication using NBP1-44073.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 50% (v/v) Glycerol with 1 mg/ml Bovine Serum Albumin (BSA)
Preservative
No Preservative
Concentration
1.07 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ARHGAP22 [p Ser397] Antibody

  • Rho GTPase activating protein 22
  • rho GTPase-activating protein 22
  • RhoGAP2
  • Rho-type GTPase-activating protein 22

Background

ARHGAP22 is a Rho GTPase-activating protein involved in the signal transduction pathway that regulates endothelial cell capillary tube formation during angiogenesis. It acts as a GTPase activator for RAC1 by converting it to an inactive GDP-bound state and also inhibits RAC1-dependent lamellipodia formation. It may also play a role in transcription regulation via its interaction with VEZF1, by regulating activity of the endothelin-1 (EDN1) promoter.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ARHGAP22 Antibody (NBP1-44073)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ARHGAP22
Entrez
Uniprot