Argonaute 3 Antibody (8E0O6)

Western Blot: Argonaute 3 Antibody (8E0O6) [NBP3-15939] - Western blot analysis of extracts of various cell lines, using Argonaute 3 Rabbit mAb (NBP3-15939) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Immunohistochemistry-Paraffin: Argonaute 3 Antibody (8E0O6) [NBP3-15939] - Immunohistochemistry of paraffin-embedded human lung cancer using Argonaute 3 Rabbit mAb (NBP3-15939) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 8E0O6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Argonaute 3 Antibody (8E0O6) Summary

• Description: Novus Biologicals Rabbit Argonaute 3 Antibody (8E0O6) (NBP3-15939) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 721-860 of human Argonaute 3 (Q9H9G7). RTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDPQALAKAVQIHQDTLRTMYFA
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AGO3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Argonaute 3 Antibody (8E0O6)

• AGO3argonaute 3
• argonaute3
• eIF2C 3
• eIF-2C 3
• Eukaryotic translation initiation factor 2C 3
• eukaryotic translation initiation factor 2C, 3
• FLJ12765
• hAgo3
• MGC86946
• protein argonaute-3

Background

Argonaute 3 encodes a member of the Argonaute family of proteins which play a role in RNA interference. The encoded protein is highly basic, contains a PAZ domain and a PIWI domain, and may play a role in short-interfering-RNA-mediated gene silencing. This gene is located on chromosome 1 in a tandem cluster of closely related family members including argonaute 4 and eukaryotic translation initiation factor 2C, 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AGO3