MAEL Antibody - BSA Free

Western Blot: MAEL Antibody [NBP1-77139] - HeLa cell lysate with MAEL antibody at 1 ug/mL.

Immunocytochemistry/Immunofluorescence: MAEL Antibody [NBP1-77139] - HeLa cells with MAEL antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: MAEL Antibody - BSA Free [NBP1-77139] - Immunofluorescence of MAEL in HeLa cells with MAEL antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

MAEL Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MAEL Antibody - BSA Free (NBP1-77139) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human Maelstrom. The immunogen is located within amino acids 30 - 80 of Maelstrom. Amino Acid Squence: REEEKEKYAEMAREWR
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAEL
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 5-20 ug/ml
  • Western Blot 1 ug/ml
• Control Peptide:

  MAEL Peptide (NBP1-77139PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Porcine (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for MAEL Antibody - BSA Free

• cancer/testis antigen 128
• CT128
• FLJ14904
• maelstrom homolog (Drosophila)
• protein maelstrom homolog
• RP11-102C16.1

Background

The mammalian homolog of the Drosophila protein Maelstrom is expressed in the male germline and localizes to the sex body in spermatocytes and the chromatoid body in round spermatids. Similar to its expression in Drosophila, Maelstrom is a component of nuages, a germ-cell specific organelle and is thought to be essential for spermatogenesis and transposon repression during meiosis. In humans, Maelstrom has been found to be expressed only in the testis and in various cancer cell lines. Treatment of these cell lines with the demethylating agent 5'-Aza-2-Deoxycytidine significantly upregulated Maelstrom levels, indicating that its expression is regulated by DNA methylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAEL
• Uniprot:
  • AAY82463(Human)