MAEL Antibody

Images

 
Western Blot: MAEL Antibody [NBP1-77139] - HeLa cell lysate with MAEL antibody at 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: MAEL Antibody [NBP1-77139] - Immunofluorescence of Maelstrom in HeLa cells with Maelstrom antibody at 20 ug/mL.
Immunocytochemistry/ Immunofluorescence: MAEL Antibody [NBP1-77139] - HeLa cells with MAEL antibody at 5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MAEL Antibody Summary

Immunogen
Antibody was raised against a 16 amino acid synthetic peptide near the carboxy terminus of human Maelstrom. The immunogen is located within amino acids 30 - 80 of Maelstrom.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAEL
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:2000
Control Peptide
MAEL Peptide (NBP1-77139PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAEL Antibody

  • cancer/testis antigen 128
  • CT128
  • FLJ14904
  • maelstrom homolog (Drosophila)
  • protein maelstrom homolog
  • RP11-102C16.1

Background

The mammalian homolog of the Drosophila protein Maelstrom is expressed in the male germline and localizes to the sex body in spermatocytes and the chromatoid body in round spermatids. Similar to its expression in Drosophila, Maelstrom is a component of nuages, a germ-cell specific organelle and is thought to be essential for spermatogenesis and transposon repression during meiosis. In humans, Maelstrom has been found to be expressed only in the testis and in various cancer cell lines. Treatment of these cell lines with the demethylating agent 5'-Aza-2-Deoxycytidine significantly upregulated Maelstrom levels, indicating that its expression is regulated by DNA methylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAEL
Uniprot