Argininosuccinate Lyase Antibody (4C5-1F2)

Product Discontinued

Argininosuccinate Lyase Antibody (4C5-1F2) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: ASL (AAH08195, 1 a.a. - 464 a.a.) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAELLFWASLCMTHLSRMAEDLILYCTKEFSFVQPSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA
• Specificity: ASL - argininosuccinate lyase
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: ASL
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot
• Application Notes: This antibody reactivity against cell lysate and recombinant protein for WB. It has been useful for IF and ELISA. IHC-Fr usage reported by a customer review.

Reactivity Notes

Rat reactivity reported from customer review.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4)
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Argininosuccinate Lyase Antibody (4C5-1F2)

• argininosuccinase
• argininosuccinate lyase
• arginosuccinase
• ASAL
• EC 4.3.2.1

Background

This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Argininosuccinate Lyase Antibody (H00000435-M01)(2)

Publications using H00000435-M01

• Huang HL, Chen WC, Hsu HP et al. Silencing of argininosuccinate lyase inhibits colorectal cancer formation. Oncol Rep 2017-01-01 [PMID: 27840980]
• Syed N, Langer J, Janczar K et al. Epigenetic status of argininosuccinate synthetase and argininosuccinate lyase modulates autophagy and cell death in glioblastoma. Cell Death Dis. 2013-02-25 [PMID: 23328665]

Reviews for Argininosuccinate Lyase Antibody (H00000435-M01) (2)

Average Rating: 4.5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Other.

reviewed by: Verified Customer IHC-Fr Other 05/27/2009

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Recombinant protein
• Species: Other
• Lot: 08221-S1

reviewed by: Verified Customer WB Other 03/30/2009

Summary

• Application: Western Blot
• Sample Tested: Anti ASL MAb, Sample Amount: 1:2000 diln
• Species: Other
• Lot: 08221S1

Bioinformatics

• Gene Symbol: ASL
• Entrez:
  • 435(Human)
• OMIM:
  • 207900(Human)
• Uniprot:
  • AAH08195(Human)