ARFGAP2 Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: ARFGAP2 Antibody [NBP1-83688] - Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.

Staining of human gastrointestinal, kidney, pancreas and placenta using Anti-ARFGAP2 antibody NBP1-84111 (A) shows similar protein distribution across tissues to independent antibody NBP1-83687 (B).

Staining of human colon shows strong cytoplasmic positivity in glandular cells.

Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Staining of human pancreas shows moderate cytoplasmic positivity in exocrine glandular cells.

• Reactivity: Hu
• Applications: ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ARFGAP2 Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: IDNMSSAVPNHSPEKKDSDFFTEHTQPPAWDAPATEPSGTQQPAPSTESSGLAQPEHGPNTDLLGTSPKASLELKSSIIGKKKPAAAKKGLGAKKGLGAQKVSSQ
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ARFGAP2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  ARFGAP2 Protein (NBP1-83688PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for ARFGAP2 Antibody - BSA Free

• ADP-ribosylation factor GTPase activating protein 2
• ADP-ribosylation factor GTPase-activating protein 2
• ARF GAP 2
• FLJ14576
• FLJ26000
• IRZ
• putative protein product of Nbla10535
• Zfp289
• Zinc finger protein 289
• zinc finger protein 289, ID1 regulated
• ZNF289GTPase-activating protein ZNF289

Background

ADP-ribosylation factor GTPase activating protein 2 (ArfGAP2) is a GTPase activating protein that interacts with the GTPase ADP-ribosylation factor (Arf) in Golgi membranes and vesicles. Although they are differentially regulated, ArfGAP2 along with ArfGAP1 and ArfGAP3 has been shown to play an overlapping role in COPI-mediated transport and in the maintenance of Golgi organization. ArfGAP2 is also known as zinc finger protein 289 or ZNF289.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ARFGAP2