Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free

Immunocytochemistry /Immunofluorescence: Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free [NBP3-43560] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells on Shi-fix coverslips stained with NBP3-43560 (1:100 dilution) for 1h followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom: NBP3-43560, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.

Western Blot: Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free [NBP3-43560] - Unboiled (A) and boiled (B) mouse skeletal muscle tissue lysates (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43560 at 1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

• Reactivity: Hu, Mu
• Applications: ELISA, Flow, IP, B/N
• Clone: D15107
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free Summary

• Description: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The original antibody was generated by immunizing an aquaporin 4 knock out mice with human AQP4 as an immunogen expressed on the surface of baculovirus. (Uniprot# P55087)
• Specificity: This antibody recognizes and binds the extracellular domains of both the M1 and M23 isoforms of human aquaporin 4 (AQP4). It also binds the M23 isoform of mouse aquaporin 4, and does not recognize the M1 isoform of mouse aquaporin 4. AQP4 is a water channel expressed on astrocytes throughout the CNS and in skeletal muscle and some epithelial cells outside of the CNS. It plays an important role in brain water homeostasis and in glymphatic solute transport. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system (CNS) that is associated with demyelination and neurological deficits in optic nerve, spinal cord and brainstem. Most NMOSD patients are seropositive for IgG autoantibodies (NMO-IgG) directed against extracellular epitopes of aquaporin-4 (AQP4). Prevention of the binding of NMO-IgG, therefore, may alleviate the disease.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: AQP4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunoprecipitation
  • Neutralization

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free

• AQP4
• AQP-4
• Aquaporin 4
• MGC22454
• MIWC
• WCH4

Background

The Aquaporin-4 gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selectivechannels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Twoalternatively spliced tra

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AQP4