Aquaporin-4 Antibodies: Novus Biologicals

NBP1-87679

Aquaporin-4 Antibody - BSA Free

(2 Reviews)

(24 Publications)

0.1 ml

NBP1-87679

Immunohistochemistry: Aquaporin-4 Antibody [NBP1-87679] - Staining of mouse cerebellum shows positivity in astrocytes, mainly in the molecular layer.

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human cerebellum shows moderate to strong membranous positivity in astrocytes, mainly in the molecular layer.

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human liver shows no positivity in hepatocytes, as expected.

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP1-87679] - Staining of human stomach shows strong membranous positivity in glandular cells.

Induction of atrogin 1 mRNA and protein expression by rotator cuff tear (RCT). (a) Expression of transcripts encoding the E3 ubiquitin-protein ligases atrogin 1, MuRF1, and UBR2 in injured muscle after RCT (n = 12 per group). (b) Atrogin 1 protein expression in muscle isolated at different stages after RCT (n = 7 per group). Densitometric analyses of the western blots are shown on the right. Data represent means +/- standard errors of the means. *P < 0.05. (c) Immunofluorescence (IF) microscopy analyses of muscle isolated at different stages after RCT. The red signal represents atrogin 1 protein. The negative control (secondary antibody only) for the IF showed no specific signal (data not shown). (d) Loss of AQP4 protein by overexpression of atrogin 1. C2C12 cells were treated with an adenovirus expressing atrogin 1 [Ad-atrogin 1; multiplicity of infection (MOI) = 50, 100, 200] for 48 h, and western blot analysis was performed with each designated antibody. (e) Monomeric ubiquitin protein expression in muscle cells isolated at different stages after RCT. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32843684), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Aquaporin levels in the sulfatide deficient mouse brain. (A) Expression of AQP1 and AQP4 in Cre+ and Cre− cerebrum samples were measured by Western Blot and quantified (B,C). Multiple AQP4 bands with different levels of glycosylation were observed (bracket). (D) Representative immunofluorescence image for AQP4 (red) in the CST cKO mouse brain at 20 months of age. Scale bar: 250 μm. (E) AQP4 immunofluorescent area around the lateral ventricle was quantified using Image J. Unpaired two-tailed t-test (normality and equal variance were assumed/confirmed). ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36613677), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Increased levels of HMGB1 protein in muscle injured by rotator cuff tear (RCT). (a) Expression level of HMGB1 protein in muscle isolated at different stages after RCT (n = 7 per group). Densitometric analyses of the western blots are shown on the right. Data represent means +/- standard errors of the means. *P < 0.05. (b) Immunofluorescence (IF) microscopy analyses of muscle cells isolated at different stages after RCT. The green signal represents HMGB1 protein. The negative control (secondary antibody only) for the IF showed no specific signal (data not shown). (c) Expression of atrogin 1 mRNA in C2C12 myotubes treated with 200 ng/mL recombinant mouse HMGB1 for the indicated time periods. (d) Atrogin 1, ubiquitin, and AQP4 protein levels in C2C12 myotubes treated with 200 ng/mL HMGB1 and an adenovirus expressing atrogin 1 [Ad-atrogin 1; multiplicity of infection (MOI) = 100] for 48 h. (e) Ubiquitination of AQP4 caused by HMGB1 and atrogin 1. C2C12 myotubes were treated with 200 ng/mL HMGB1 and Ad-atrogin 1 (MOI = 100) with or without MG132. After 48 h of incubation, cell lysates were prepared for a ubiquitination assay using anti-AQP4 and anti-ubiquitin antibodies. (f) Expression of atrogin 1 mRNA in C2C12 myotubes exposed to 5 μM SB203580 (a p38 MAPK inhibitor), LY294002 (a PI3K inhibitor), BAY 11-7082 (an NF-kappa B inhibitor), PD98059 (an MEK inhibitor), or SP600125 (a JNK inhibitor). Cells were exposed to each inhibitor for 1 h prior to treatment with 200 ng/mL HMGB1 for 24 h. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32843684), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reduced aquaporin 4 (AQP4) protein levels in atrophied muscle following rotator cuff tear (RCT). (a) Reduced myofiber size in muscle injured by RCT. Magnified view of the H&E-stained muscle. Magnification, × 400. Scale bar, 50 μm. The yellow dotted line indicates the muscle myofiber. (b) Myofiber size in control (CTL) and RCT muscles. The size of each dotted area was measured using ImageJ software. *P < 0.05. (c) Western blot analyses for myosin heavy chain (MYH) protein expression between control and the injured muscle after RCT (n = 7 per group). Densitometric analyses of the western blots are shown in the graphs. Data represents mean +/- SEM. *p < 0.05. (d) Reduced aquaporin 4 (AQP4) protein levels in atrophied muscle following RCT (n = 7 per group). Densitometric analyses of the western blots are shown on the right. Data represent means +/- standard errors of the means. *P < 0.05. (e) Immunofluorescence microscopy analyses of muscle cells isolated at different stages after RCT (n = 3 per group). The green signal in each panel represents membranous AQP4 protein and the blue signal represents cell nuclei. Magnification, × 200. Scale bar, 100 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/32843684), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western blot results. (A) Western blot analysis of SNTA1 and AQP4 protein expression in Group C, Group PDN, and Group BHB. (B) Relative protein expression of SNTA1 and AQP4. Due to the reason that some blots in the original saved images were not mentioned in the present study, we spliced the blots and the typical ones were chosen to indicate the differences in the expression of proteins. Refer to Figure 1 for the meaning of the grouping abbreviation. Values were presented as mean +/- SEM. *p < 0.05 compared with values in Group PDN. #p < 0.05 compared with values in Group C. SNTA1 means alpha -syntrophin, and AQP4 means aquaporin-4 protein. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35898407), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-87679-25ul

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NBP3-35055

Aquaporin-4 Antibody (5A4W4)

100 ul

NBP3-35055

Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Aquaporin-4 Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (5A4W4) [NBP3-35055] - Human lung cancer tissue using Aquaporin-4 (AQP4) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP3-44612

Aquaporin-4 Antibody (AQP4/3323)

100 μg

NBP3-44612

Western Blot Analysis of HepG2 cell lysate using Aquaporin-4 Antibody (AQP4/3323).

Flow Cytometric Analysis of PFA-fixed Ramos cells using Aquaporin-4 Antibody (AQP4/3323). Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Analysis of Protein Array containing more than 19,000 full-length human proteins using Aquaporin-4 Antibody (AQP4/3323).

Formalin-fixed, paraffin-embedded human small intestine stained with Aquaporin-4 Antibody (AQP4/3323)). HIER: Tris/EDTA, pH9.0, 45min. 2 : HRP-polymer, 30min. DAB, 5min.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-45054

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NBP2-92886

Aquaporin-4 Antibody - Azide and BSA Free

(2 Publications)

0.1 ml

NBP2-92886

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP2-92886] - Immunohistochemistry of paraffin-embedded mouse kidney using Aquaporin-4 Rabbit pAb (NBP2-92886) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence: Aquaporin-4 Antibody - Azide and BSA Free [NBP2-92886] - Immunofluorescence analysis of paraffin-embedded mouse brain using Aquaporin-4 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Aquaporin 4 (AQP4) abundance is decreased in dystrophin-deficient diaphragms. (A) Ingenuity pathway analysis (IPA) demonstrated changes in various dystrophin-associated proteins, including a decrease in the level of AQP4, which was confirmed by (B) Western blot (Figure S2) and its densitometric analysis, n = 5/group; results are shown as mean +/- SEM; ** p < 0.01. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/38002330), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-92886-0.02ml

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NBP3-12902

Aquaporin-4 Antibody

100 ul

NBP3-12902

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Aquaporin-4 stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein at cell membrane in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. Aquaporin-4 antibody (NBP3-12902) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Frozen: Aquaporin-4 Antibody [NBP3-12902] - Aquaporin-4 antibody detects Aquaporin-4 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: Aquaporin-4 protein stained by Aquaporin-4 antibody (NBP3-12902) diluted at 1:250. Red: NeuN, stained by NeuN antibody [2Q158] diluted at 1:500. Blue: Fluoroshield with DAPI.

Reactivity:

Mu, Rt, Hu, Po, Bv, RM, Sh

Species Glossary

Reactivity: Mu, Rt, Hu, Po, Bv, RM, Sh

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-52872

Aquaporin-4 Antibody (CL0178) - BSA Free

0.1 ml

NBP2-52872

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human cerebellum shows strong positivity in neuropil, mainly in the molecular layer.

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human stomach shows moderate to strong membranous positivity in glandular cells.

Immunohistochemistry-Paraffin: Aquaporin-4 Antibody (CL0178) [NBP2-52872] - Staining of human liver shows no positivity in hepatocytes as expected.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-43897AF350
Alexa Fluor 405	NBP3-43897AF405
Alexa Fluor 488	NBP3-43897AF488
Alexa Fluor 532	NBP3-43897AF532
Alexa Fluor 594	NBP3-43897AF594
Alexa Fluor 647	NBP3-43897AF647
Alexa Fluor 700	NBP3-43897AF700
Alexa Fluor 750	NBP3-43897AF750
Alexa Fluor Plus 405	NBP3-43897AFP405
Alexa Fluor Plus 488	NBP3-43897AFP488
Alexa Fluor Plus 555	NBP3-43897AFP555
Alexa Fluor Plus 594	NBP3-43897AFP594
Alexa Fluor Plus 647	NBP3-43897AFP647
Alexa Fluor Plus 680	NBP3-43897AFP680
Allophycocyanin	NBP3-43897APC
Biotin	NBP3-43897B
CoraFluor 1	NBP3-43897CL1
DyLight 350	NBP3-43897UV
DyLight 405	NBP3-43897V
DyLight 488	NBP3-43897G
DyLight 550	NBP3-43897R
DyLight 594	NBP3-43897DL594
DyLight 650	NBP3-43897C
DyLight 680	NBP3-43897FR
DyLight 755	NBP3-43897IR
FITC	NBP3-43897F
HRP	NBP3-43897H
Janelia Fluor 525	NBP3-43897JF525
Janelia Fluor 549	NBP3-43897JF549
Janelia Fluor 646	NBP3-43897JF646
Janelia Fluor 669	NBP3-43897JF669
PE	NBP3-43897PE
PerCP	NBP3-43897PCP
mFluor Violet 450 SE	NBP3-43897MFV450
mFluor Violet 500 SE	NBP3-43897MFV500
mFluor Violet 610 SE	NBP3-43897MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-43897
BSA Free	NBP2-52872-25ul

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MAB11659

Aquaporin-4 Antibody (1093722) [Unconjugated]

(1 Publication)

100 μg

MAB11659

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the brain astrocytes. Protocol available in COMET™ Panel Builder." class="big_lightbox">

AQP4 was detected in immersion fixed paraffin-embedded sections of human brain Cortex using Mouse Anti-Human AQP4, Monoclonal Antibody (Catalog #MAB11659) at 25ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the brain astrocytes. Protocol available in COMET™ Panel Builder." alt="AQP4 was detected in immersion fixed paraffin-embedded sections of human brain Cortex using Mouse Anti-Human AQP4, Monoclonal Antibody (Catalog #MAB11659) at 25ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 8 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the brain astrocytes. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of astrocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

Aquaporin 4/AQP4 was detected in immersion fixed paraffin-embedded sections of human brain cortex using Mouse Anti-Human Aquaporin 4/AQP4 Monoclonal Antibody (Catalog # MAB11659) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface of astrocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB11659U-100UG
Alexa Fluor 405	FAB11659V-100UG
Alexa Fluor 488	FAB11659G-100UG
Alexa Fluor 532	FAB11659X-100UG
Alexa Fluor 594	FAB11659T-100UG
Alexa Fluor 647	FAB11659R-100UG
Alexa Fluor 700	FAB11659N-100UG
Alexa Fluor 750	FAB11659S-100UG
Alexa Fluor Plus 405	FAB11659AFP405-100UG
Alexa Fluor Plus 488	FAB11659AFP488-100UG
Alexa Fluor Plus 555	FAB11659AFP555-100UG
Alexa Fluor Plus 594	FAB11659AFP594-100UG
Alexa Fluor Plus 647	FAB11659AFP647-100UG
Alexa Fluor Plus 680	FAB11659AFP680-100UG
Allophycocyanin	FAB11659A
Biotin	FAB11659B
CoraFluor 1	FAB11659CL1
DyLight 350	FAB11659D
DyLight 405	FAB11659E
DyLight 488	FAB11659K
DyLight 550	FAB11659L
DyLight 594	FAB11659M
DyLight 650	FAB11659W
DyLight 680	FAB11659Y
DyLight 755	FAB11659Z
FITC	FAB11659F
HRP	FAB11659H
Janelia Fluor 525	FAB11659JF525
Janelia Fluor 549	FAB11659I
Janelia Fluor 646	FAB11659J
Janelia Fluor 669	FAB11659JF669
PE	FAB11659P
PerCP	FAB11659C
mFluor Violet 450 SE	FAB11659MFV450
mFluor Violet 500 SE	FAB11659MFV500
mFluor Violet 610 SE	FAB11659MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11659-SP

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NBP3-52095

Aquaporin-4 Antibody (CL0178)

100 ul

NBP3-52095

Staining of human stomach shows moderate to strong membranous positivity in glandular cells.

Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.

Analysis in human cerebral cortex and liver tissues. Corresponding Aquaporin-4 RNA-seq data are presented for the same tissues.

Staining of human cerebellum shows strong positivity in neuropil, mainly in the molecular layer.

Analysis in human cerebral cortex tissue.

Staining of human liver shows no positivity in hepatocytes as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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MAB11748

Aquaporin-4 Antibody (3366C) [Unconjugated]

100 μg

MAB11748

DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the brain astrocytes. Protocol available in COMET™ Panel Builder." class="big_lightbox">

AQP4 was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-Human AQP4, Monoclonal Antibody (Catalog #MAB11748) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH)Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the brain astrocytes. Protocol available in COMET™ Panel Builder." alt="AQP4 was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-Human AQP4, Monoclonal Antibody (Catalog #MAB11748) at 10ug/mL at 37 ° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH)Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the brain astrocytes. Protocol available in COMET™ Panel Builder." class="big_thumb" />

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Aquaporin 4/AQP4 was detected in immersion fixed paraffin-embedded sections of human brain cortex using Rabbit Anti-Human Aquaporin 4/AQP4 Monoclonal Antibody (Catalog # MAB11748) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # <a class=

VCTS021). Tissue was stained using the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to astrocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11748-SP

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NBP3-43560

Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free

0.2 mg

NBP3-43560

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NBP1-97928

Aquaporin-4 Antibody - BSA Free

0.1 ml

NBP1-97928

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-97928-0.025ml

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NBP3-13952

Aquaporin-4 Antibody (AQP4/3324)

100 μg

NBP3-13952

Protein Array: Aquaporin-4 Antibody (AQP4/3324) [NBP3-13952] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Aquaporin-4 Antibody (AQP4/3324).

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-14104AF350
Alexa Fluor 405	NBP3-14104AF405
Alexa Fluor 488	NBP3-14104AF488
Alexa Fluor 532	NBP3-14104AF532
Alexa Fluor 594	NBP3-14104AF594
Alexa Fluor 647	NBP3-14104AF647
Alexa Fluor 700	NBP3-14104AF700
Alexa Fluor 750	NBP3-14104AF750
Alexa Fluor Plus 405	NBP3-14104AFP405
Alexa Fluor Plus 488	NBP3-14104AFP488
Alexa Fluor Plus 555	NBP3-14104AFP555
Alexa Fluor Plus 594	NBP3-14104AFP594
Alexa Fluor Plus 647	NBP3-14104AFP647
Alexa Fluor Plus 680	NBP3-14104AFP680
Allophycocyanin	NBP3-14104APC
Biotin	NBP3-14104B
CoraFluor 1	NBP3-14104CL1
DyLight 350	NBP3-14104UV
DyLight 405	NBP3-14104V
DyLight 488	NBP3-14104G
DyLight 550	NBP3-14104R
DyLight 594	NBP3-14104DL594
DyLight 650	NBP3-14104C
DyLight 680	NBP3-14104FR
DyLight 755	NBP3-14104IR
FITC	NBP3-14104F
HRP	NBP3-14104H
Janelia Fluor 525	NBP3-14104JF525
Janelia Fluor 549	NBP3-14104JF549
Janelia Fluor 646	NBP3-14104JF646
Janelia Fluor 669	NBP3-14104JF669
PE	NBP3-14104PE
PerCP	NBP3-14104PCP
mFluor Violet 450 SE	NBP3-14104MFV450
mFluor Violet 610 SE	NBP3-14104MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-14104

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

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Protein/Target

Applications

Reactivity

Citation

Reviews

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Available Conjugates

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Clone

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Has Sample Size

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Species Glossary

Aquaporin-4 Antibodies

Aquaporin-4 Antibody - BSA Free

Aquaporin-4 Antibody (5A4W4)

Aquaporin-4 Antibody (AQP4/3323)

Aquaporin-4 Antibody - Azide and BSA Free

Aquaporin-4 Antibody

Aquaporin-4 Antibody (CL0178) - BSA Free

Aquaporin-4 Antibody (1093722) [Unconjugated]

Aquaporin-4 Antibody (CL0178)

Aquaporin-4 Antibody (3366C) [Unconjugated]

Aquaporin-4 Antibody (D15107) - Chimeric - BSA Free

Aquaporin-4 Antibody - BSA Free

Aquaporin-4 Antibody (AQP4/3324)