Aquaporin-4 Antibody (CL0178) - Azide and BSA Free

Analysis in human cerebral cortex and liver tissues using NBP3-43897 antibody. Corresponding Aquaporin-4 RNA-seq data are presented for the same tissues.

Staining of human liver shows no positivity in hepatocytes as expected.

Staining of human cerebral cortex shows moderate to strong membranous positivity in astrocytes.

Staining of human cerebellum shows strong positivity in neuropil, mainly in the molecular layer.

Analysis in human cerebral cortex tissue.

Staining of human stomach shows moderate to strong membranous positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0178
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Aquaporin-4 Antibody (CL0178) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Aquaporin-4 Antibody (CL0178) - Azide and BSA Free (NBP2-52872) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P55087, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: AQP4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:500 - 1:1000
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for Aquaporin-4 Antibody (CL0178) - Azide and BSA Free

• AQP4
• AQP-4
• Aquaporin 4
• MGC22454
• MIWC
• WCH4

Background

The Aquaporin-4 gene encodes a member of the aquaporin family of intrinsic membrane proteins that function as water-selectivechannels in the plasma membranes of many cells. The encoded protein is the predominant aquaporin found in brain. Twoalternatively spliced tra

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: AQP4