Aquaporin-2 [p Ser261] Antibody

Images

 
Western Blot: Aquaporin-2 [p Ser261] Antibody [NB100-61100] - Rat kidney lysate showing specific immunolabeling of the ~ 29k and 37k glycosylated form of the AQP2 protein phosphorylated at Ser261. Immunolabeling is ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, PmSpecies Glossary
Applications WB, DB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Aquaporin-2 [p Ser261] Antibody Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phosphoSer261of rat aquaporin 2.
Modification
p Ser261
Specificity
Specific for ~29k AQP2 protein phosphorylated at Ser261. Also recognizes the glycosylated form of AQP2 at ~ 37k. Immunolabeling of the AQP2 band is blocked by preadsorption with the phospho-peptide used as antigen but not by the corresponding dephospho-peptide.
Predicted Species
Primate (100%), Chicken (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AQP2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Dot Blot
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
Immunohistochemistry as reported in the literature (Hoffert et al., 2007) Dot Blot was reported in scientific literature.
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NB100-61100 in the following applications:

Publications
Read Publications using
NB100-61100 in the following applications:

  • DB
    1 publication
  • WB
    2 publications

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 25977473)

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for Aquaporin-2 [p Ser261] Antibody

  • ADH water channel
  • AQP-2
  • AQP-CD
  • aquaporin 2 (collecting duct)
  • aquaporin-2
  • aquaporin-CD
  • Collecting duct water channel protein
  • MGC34501
  • Water channel protein for renal collecting duct
  • water-channel aquaporin 2
  • WCH-CD

Background

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein aquaporins. Aquaporin 0, and Aquaporin 1 have been the foundation of the growing family of aquaporins. The lens specific Aquaporin 0 represents up to 80% of total lens membrane protein. Defects in Aquaporin 0 are a cause of autosomal dominant cataract. The lens opacity mutation is an AA substitution that inhibits targeting of MIP to the cell membrane. Human Aquaporin 0 is a 263 amino acid transmembrane protein belonging to the MIP family. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C terminus are predicted to be cytoplasmic. Aquaporin 2 is located in the collecting tubule.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Aquaporin-2 Antibody (NB100-61100)(2)

We have publications tested in 2 confirmed species: Human, Canine.

We have publications tested in 2 applications: DB, WB.


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DB
(1)
WB
(2)
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(1)
Canine
(1)
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Review for Aquaporin-2 Antibody (NB100-61100) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-61100:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot Aquaporin-2 NB100-61100
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reviewed by:
Grazia Tamma
WB Human 04/06/2016
View

Summary

ApplicationWestern Blot
SpeciesHuman
FileView PDF

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FAQs for Aquaporin-2 Antibody (NB100-61100) (0)

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Recent Reviews

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2
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1
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Grazia Tamma
04/06/2016
Application: WB
Species: Human

Bioinformatics

Gene Symbol AQP2
Entrez
Uniprot