APPBP1 Overexpression Lysate

Images

 
Western Blot: NAE1 Overexpression Lysate (Adult Normal) [NBL1-13459] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAE1.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

APPBP1 Overexpression Lysate Summary

Description

APPBP1 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC201326

Accession#: NM_001018160

Protein Tag: C-MYC/DDK
Gene
NAE1

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
50.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for APPBP1 Overexpression Lysate

  • A-116A10.1
  • amyloid beta precursor protein binding protein 1, 59kDa
  • Amyloid beta precursor protein-binding protein 1, 59 kDa
  • amyloid beta precursor protein-binding protein 1, 59kD
  • APPBP1
  • APP-BP1
  • HPP1
  • NAE1
  • NEDD8 activating enzyme E1 subunit 1
  • NEDD8-activating enzyme E1 regulatory subunit
  • NEDD8-activating enzyme E1 subunit
  • protooncogene protein 1
  • Proto-oncogene protein 1
  • ula-1

Background

The protein encoded by this gene binds to the beta-amyloid precursor protein. Beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. In addition, the encoded protein can form a heterodimer with UBE1C and bind and activate NEDD8, a ubiquitin-like protein. This protein is required for cell cycle progression through the S/M checkpoint. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]. Transcript Variant: This variant (3) lacks an alternate exon compared to variant 1. The resulting isoform (c) is shorter at the N-terminus compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for NEDD8 Activating Enzyme (APPBP1/UBA3) Lysate (NBL1-13459) (0)

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Bioinformatics

Gene Symbol NAE1