Apolipoprotein L2 Antibody - BSA Free

Western Blot: Apolipoprotein L2 Antibody [NBP2-98660] - Anti-Apolipoprotein L2 rabbit polyclonal antibody at 1:500 dilution. Lane A: U-251 MG Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 37 kDa (We are unsure as to the identity of these extra bands).

Immunohistochemistry-Paraffin: Apolipoprotein L2 Antibody [NBP2-98660] - Immunochemical staining of human Apolipoprotein L2 in human urinary bladder with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry-Paraffin: Apolipoprotein L2 Antibody [NBP2-98660] - Immunochemical staining of human Apolipoprotein L2 in human brain with rabbit polyclonal antibody at 1:500 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: Apolipoprotein L2 Antibody [NBP2-98660] - Apolipoprotein L2 was immunoprecipitated using: Lane A: 0.5 mg U-251MG Whole Cell Lysate. 4 ul anti-Apolipoprotein L2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Apolipoprotein L2 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 37 kDa. Observed band size: 40 kDa

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Apolipoprotein L2 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human Apolipoprotein L2 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: APOL2
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for Apolipoprotein L2 Antibody - BSA Free

• APOL3
• APOL-II
• apolipoprotein L, 2
• apolipoprotein L2
• Apolipoprotein L-II

Background

Apolipoprotein L2 is a member of the apolipoprotein L gene family. The encoded protein is found in the cytoplasm, where it may affect the movement of lipids or allow the binding of lipids to organelles. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOL2