Apolipoprotein L4 Antibody

Western Blot: Apolipoprotein L4 Antibody [NBP1-20883] - Analysis of Apolipoprotein L4 in Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using this antibody at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: Apolipoprotein L4 Antibody [NBP1-20883] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Apolipoprotein L4 Antibody Summary

• Description: Novus Biologicals Goat Apolipoprotein L4 Antibody (NBP1-20883) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Apolipoprotein L4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ENLNELTHIHQSLK corresponding to C-Terminus according to NP_085146.2, NP_663693.1.
• Epitope: C terminus
• Specificity: This antibody is expected to recognize both reported isoforms (NP_085146.2 and NP_663693.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOL4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1:1000
• Application Notes: WB: Approx. 37 kDa band observed in human placenta lysates (calculated MW of 38.7 kDa band according to NP_085146.2). Two additional bands of unknown identity were also consistently observed at 50 kDa band and at 70 kDa. The band at 50 kDa band was successfully blocked by incubation with the immunizing peptide. The other band at 70 kDa band however was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when the antibody is used for other assays than WB.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein L4 Antibody

• apolipoprotein L, 4
• apolipoprotein L4
• Apolipoprotein L-IV
• APOLIV
• APOL-IV

Background

The protein encoded by this gene is a member of the apolipoprotein L family and may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Two transcript variants encoding two different isoforms have been found for this gene. Only one of the isoforms appears to be a secreted protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein L4 Antibody (NBP1-20883)(1)

Publication using NBP1-20883

• Monajemi H, Fontijn RD, Pannekoek H, Horrevoets AJ. The apolipoprotein L gene cluster has emerged recently in evolution and is expressed in human vascular tissue. Genomics 2002-04-01 [PMID: 11944986]

Bioinformatics

• Gene Symbol: APOL4
• Entrez:
  • 80832(Human)
• Uniprot:
  • NP_085146.2(Human)