Apolipoprotein L4 Antibody

Images

 
Western Blot: Apolipoprotein L4 Antibody [NBP1-20883] - Analysis of Apolipoprotein L4 in Human Placenta lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide using this ...read more
Immunohistochemistry-Paraffin: Apolipoprotein L4 Antibody [NBP1-20883] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Apolipoprotein L4 Antibody Summary

Immunogen
Peptide with sequence C-ENLNELTHIHQSLK corresponding to C-Terminus according to NP_085146.2, NP_663693.1.
Epitope
C terminus
Specificity
This antibody is expected to recognize both reported isoforms (NP_085146.2 and NP_663693.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APOL4
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
Application Notes
WB: Approx. 37 kDa band observed in human placenta lysates (calculated MW of 38.7 kDa band according to NP_085146.2). Two additional bands of unknown identity were also consistently observed at 50 kDa band and at 70 kDa. The band at 50 kDa band was successfully blocked by incubation with the immunizing peptide. The other band at 70 kDa band however was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when the antibody is used for other assays than WB.
Publications
Read Publication using NBP1-20883.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris (pH 7.3), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Apolipoprotein L4 Antibody

  • apolipoprotein L, 4
  • apolipoprotein L4
  • Apolipoprotein L-IV
  • APOLIV
  • APOL-IV

Background

The protein encoded by this gene is a member of the apolipoprotein L family and may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Two transcript variants encoding two different isoforms have been found for this gene. Only one of the isoforms appears to be a secreted protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Apolipoprotein L4 Antibody (NBP1-20883)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol APOL4
Entrez
Uniprot