Apolipoprotein L1 Antibody

Western Blot: Apolipoprotein L1 Antibody [NBP1-06017] - (0.1ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Apolipoprotein L1 Antibody Summary

• Description: Novus Biologicals Goat Apolipoprotein L1 Antibody (NBP1-06017) is a polyclonal antibody validated for use in WB and ELISA. Anti-Apolipoprotein L1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-NNNYKILQADQE corresponding to C-Terminus according to NP_003652.2, NP_663318.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_003652.2; NP_663318.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 45 kDa band observed in human placenta lysates (calculated MW of 44 kDa band according to NP_003652.2 and of 45.9 kDa band according to NP_663318.1).
• Theoretical MW: 44 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein L1 Antibody

• APOL
• APO-L
• APOL1
• APO-L1
• APO-LI
• APOL-I
• Apolipoprotein L
• apolipoprotein L, 1
• Apolipoprotein L1
• Apolipoprotein L-I
• FSGS4

Background

This gene encodes a secreted high density lipoprotein which binds to apolipoprotein A-I. Apolipoprotein A-I is a relatively abundant plasma protein and is the major apoprotein of HDL. It is involved in the formation of most cholesteryl esters in plasma and also promotes efflux of cholesterol from cells. This apolipoprotein L family member may play a role in lipid exchange and transport throughout the body, as well as in reverse cholesterol transport from peripheral cells to the liver. Several different transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein L1 Antibody (NBP1-06017)(1)

Publication using NBP1-06017

• Perez-Morga D, Vanhollebeke B, Paturiaux-Hanocq F et al. Apolipoprotein L-I promotes trypanosome lysis by forming pores in lysosomal membranes. Science 2005-07-15 [PMID: 16020735]

Bioinformatics

• Gene Symbol: APOL1
• Entrez:
  • 8542(Human)
• Uniprot:
  • O14791(Human)