Apolipoprotein E/ApoE Antibody (PSH02-85)

Western Blot: Apolipoprotein E/ApoE Antibody (PSH02-85) [NBP3-32045] - Western blot analysis of Apolipoprotein E/ApoE on different lysates with Rabbit anti-Apolipoprotein E/ApoE antibody (NBP3-32045) at 1/2,000 dilution. Lane 1: 293T-NT cell lysate Lane 2: 293T-OE-APOE2 cell lysate Lane 3: 293T-OE-APOE3 cell lysate Lane 4: 293T-OE-APOE4 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 36 kDa Observed band size: 36 kDa Exposure time: 2 minutes 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32045) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu
• Applications: WB
• Clone: PSH02-85
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Apolipoprotein E/ApoE Antibody (PSH02-85) Summary

• Description: Novus Biologicals Rabbit Apolipoprotein E/ApoE Antibody (PSH02-85) (NBP3-32045) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within human Apolipoprotein E/ApoE aa 101-150 / 317. (Uniprot: P02649)
• Localization: Secreted, extracellular space, extracellular matrix.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: APOE
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 36 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Apolipoprotein E/ApoE Antibody (PSH02-85)

• APOE
• Apo-E
• Apolipoprotein E
• apolipoprotein E3
• late onset)
• MGC1571

Background

Apolipoprotein E (APOE) is an apolipoprotein located in chylomicrons and intermediate-density lipoproteins that is necessary for the normal catabolism of triglyceride-rich lipoprotein constituents in the liver. APOE has recently been shown to be involseveral biological processes other than lipoprotein transport, including cognition and immunoregulation.

Lipoproteins are responsible for carrying cholesterol and other fats through the bloodstream as little packages and are essential for the normal breakdown of these molecules. In particular, apolipoprotein E is a major component of specific lipoproteins called very low-density lipoproteins (VLDL). A major function of VLDLs is to remove excess cholesterol from the blood and carry it to the liver for processing. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes.

The three Apo E isoforms are strongly associated with cardiovascular disease and Alzheimer's disease, so ApoE antibodies have become useful tools for research in both of these areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APOE