Apolipoprotein E/ApoE Antibody (1H4)

Images

 
Western Blot: ApoE Antibody (1H4) [NBP2-22110] - Western blot analysis using ApoE mAb against HEK293 (1) and ApoE (AA: 20-267)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: ApoE Antibody (1H4) [NBP2-22110] - Immunohistochemical analysis of paraffin-embedded liver cancer tissues (left) and brain tissues (right) using ApoE mouse mAb with DAB staining.
Flow Cytometry: ApoE Antibody (1H4) [NBP2-22110] - Flow cytometric analysis of HepG2 cells using ApoE mouse mAb (green) and negative control (purple).
ELISA: ApoE Antibody (1H4) [NBP2-22110] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC-P
Clone
1H4
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Apolipoprotein E/ApoE Antibody (1H4) Summary

Immunogen
Purified recombinant fragment of human ApoE expressed in E. coli. [UniProt# P02649]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
APOE
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry-Paraffin 1:200-1:1000
Application Notes
This ApoE (1H4) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
Bladder Lysate (NB820-60568)

Reactivity Notes

Human.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for Apolipoprotein E/ApoE Antibody (1H4)

  • APOE
  • Apo-E
  • Apolipoprotein E
  • apolipoprotein E3
  • late onset)
  • MGC1571

Background

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. Tissue specificity: Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL). APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APOE
Entrez
Uniprot