Mouse Apolipoprotein D ELISA Kit (Colorimetric) Summary
| Specificity |
This assay has high sensitivity and excellent specificity for detection of Mouse Apolipoprotein D. No significant cross-reactivity or interference between Mouse Apolipoprotein D and analogues was observed. |
| Standard Curve Range |
0.27 ng/mL-200 ng/mL |
| Sensitivity |
0.27 ng/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 10% |
| Intra-Assay |
CV% < 8% |
| Spike Recovery |
87-103% |
| Sample Volume |
50-100 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
APOD |
Applications/Dilutions
Packaging, Storage & Formulations
Kit Components
|
Components
|
- Adhesive Strip (For 96 wells)
- Assay Plate (12 x 8 coated Microwells)
- HRP-Conjugate (100 x concentrate)
- HRP-Conjugate Diluent
- Instruction Manual
- Sample Diluent
- Standard (Freeze dried)
- Stop Solution
- TMB Substrate
- Wash Buffer (25 x concentrate)
|
Alternate Names for Mouse Apolipoprotein D ELISA Kit (Colorimetric)
Background
The Apolipoprotein D gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoproteinsequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2microglobulin protein superfamily
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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Product General Protocols
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FAQs for Apolipoprotein D ELISA Kit (NBP2-77036). (Showing 1 - 1 of 1 FAQ).
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What is the difference between the Quantikine and Duoset ELISA kits?
- Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.
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