Apolipoprotein D Antibody

Western Blot: Apolipoprotein D Antibody [NBP2-42512] - Analysis of Human Kidney (A) and Liver (B) lysates (35 ug protein in RIPA buffer). Antibody at 0.3 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry: Apolipoprotein D Antibody [NBP2-42512] - Staining of paraffin embedded Human Adrenal Gland. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Apolipoprotein D Antibody Summary

• Description: Novus Biologicals Goat Apolipoprotein D Antibody (NBP2-42512) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Apolipoprotein D Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-ENGKIKVLNQE corresponding to internal region according to NP_001638.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: APOD
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 ug/mL
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.3 - 1 ug/mL
• Application Notes: WB: Approx. 26 kDa band observed in human liver and kidney lysates (calculated MW of 21.3 kDa band according to NP_001638.1). The observed molecular weight corresponds to the glycosylated form.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Apolipoprotein D Antibody

• ApoD
• apo-D
• apolipoprotein D

Background

The Apolipoprotein D gene encodes a component of high density lipoprotein that has no marked similarity to other apolipoproteinsequences. It has a high degree of homology to plasma retinol-binding protein and other members of the alpha 2microglobulin protein superfamily

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Apolipoprotein D Antibody (NBP2-42512)(1)

Publication using NBP2-42512

• Chen H, Li Y, Du J et al. Increased JNK1 activity contributes to the upregulation of ApoD in the apocrine secretory gland cells from axillary osmidrosis. Mol Cell Biochem 2011-08-01 [PMID: 21526344]

Bioinformatics

• Gene Symbol: APOD
• Entrez:
  • 347(Human)