Cullin 5 Antibody

Images

 
Western Blot: Cullin 5 Antibody [NBP1-22970] - Whole cell lysate (10 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 ...read more
Immunohistochemistry-Paraffin: Cullin 5 Antibody [NBP1-22970] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Cul5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Independent Antibodies: Western Blot: Cullin 5 Antibody [NBP1-22970] - by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP ...read more
Western Blot: Cullin 5 Antibody [NBP1-22970] - Whole cell lysate (50 ug) from HEK293T, LNCaP, and Hep-G2 cellsprepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Pm-Or, RbSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

Cullin 5 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human cullin-5 using the numbering given in entry NP_003469.2 (GeneID 8065).
Predicted Species
Rat (100%), Orangutan (100%), Rabbit (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CUL5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Publications
Read Publication using
NBP1-22970 in the following applications:

  • IP
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cullin 5 Antibody

  • CUL-5
  • cullin 5
  • VACM1Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1)
  • VACM-1cullin-5
  • Vasopressin-activated calcium-mobilizing receptor 1
  • Vasopressin-activated calcium-mobilizing receptor-1

Background

Cullin-5 (Cul-5) is a core component of multiple E3 ubiquitin ligase complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cullin 5 Antibody (NBP1-22970)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IP, WB.


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IP
(1)
WB
(1)
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Human
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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol CUL5
Entrez
Uniprot