Cullin 5 Antibody

Western Blot: Cullin 5 Antibody [NBP1-22970] - Whole cell lysate (10 ug) from NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: Cullin 5 Antibody [NBP1-22970] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Cul5 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Western Blot: Cullin 5 Antibody [NBP1-22970] - by Western Blot (h&m) and Immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 mcg) and mouse NIH3T3 (M; 50mcg) cells. Antibodies: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 10 mcg/mg lysate. Cul5 was also immunoprecipitated by another rabbit anti-Cul5 antibody, which recognizes a downstream epitope.

Western Blot: Cullin 5 Antibody [NBP1-22970] - Whole cell lysate (50 ug) from HEK293T, LNCaP, and Hep-G2 cellsprepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cul5 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Rt, Pm-Or, Rb
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cullin 5 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human cullin-5 using the numbering given in entry NP_003469.2 (GeneID 8065).
• Predicted Species: Rat (100%), Orangutan (100%), Rabbit (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CUL5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500- 1:2000
  • Immunohistochemistry-Paraffin 1:500 - 1:2000
  • Immunoprecipitation 5-15 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cullin 5 Antibody

• CUL-5
• cullin 5
• VACM1Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1)
• VACM-1cullin-5
• Vasopressin-activated calcium-mobilizing receptor 1
• Vasopressin-activated calcium-mobilizing receptor-1

Background

Cullin-5 (Cul-5) is a core component of multiple E3 ubiquitin ligase complexes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cullin 5 Antibody (NBP1-22970)(1)

Publication using NBP1-22970

• Weems JC, Slaughter BD, Unruh JR, Hall SM. Assembly of the Elongin A Ubiquitin Ligase Is Regulated by Genotoxic and Other Stresses JBC Papers in Press. 2015-04-15 [PMID: 25878247] (WB, IP, Human)

Bioinformatics

• Gene Symbol: CUL5
• Entrez:
  • 8065(Human)
• Uniprot:
  • NP_003469.2(Human)