APLP-2 Antibody (397702)

Western blot shows lysates of human brain (Alzheimer's disease hippocampus) tissue. PVDF Membrane was probed with 2 µg/mL of Human APLP‑2 Monoclonal Antibody (Catalog # MAB49451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for APLP‑2 at approximately 150 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB
• Clone: 397702
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

APLP-2 Antibody (397702) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human APLP-2
  Leu30-Ser680
  Accession # Q06481
• Specificity: Detects human APLP-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse APLP-2 or recombinant human APLP-1 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: APLP2
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APLP-2 Antibody (397702)

• amyloid beta (A4) precursor-like protein 2
• amyloid precursor protein homolog HSD-2
• Amyloid protein homolog
• amyloid-like protein 2
• APLP2
• APLP-2
• APPH
• APPHCDEI box-binding protein
• APPL2
• CDEBP
• WALPLP2
• YWK-II

Background

Amyloid beta precursor-like protein 2 (APLP-2) is a 120-170 kDa member of the APP family of neuronal type I transmembrane proteins. Its extracellular domain consists of an N-terminal Cys-rich domain, an Asp/Glu-rich acidic region, a Kunitz protease inhibitor (KPI) domain, and a GAG attachment site in the membrane proximal domain. APLP-2 forms both homodimers and heterodimers with APP and APLP-1. Proteolytic cleavage of APLP-2 generates peptides similar to the amyloidogenic A beta peptides and a cytoplasmic fragment that functions as a transcriptional coactivator. Alternate splicing of APLP-2 generates isoforms that lack the KPI domain or contain an insertion that prevents GAG attachment. The extracellular domain of human APLP-2 shares 81% and 93% aa sequence identity with mouse and rat APLP-2, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for APLP-2 Antibody (MAB49451)(1)

Publication using MAB49451

• DeVay R, Yamamoto L, Shelton D, Liang H Common Proprotein Convertase Subtilisin/Kexin Type 9 (PCSK9) Epitopes Mediate Multiple Routes for Internalization and Function. PLoS ONE, 2015;10(4):e0125127. 2015-01-01 [PMID: 25905719] (Western Blot, Human)

Bioinformatics

• Gene Symbol: APLP2
• Uniprot:
  • Q06481()