API5 Antibody

Western Blot: API5 Antibody [NBP1-46223] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-API5 antibody A302-784A used for WB at 0.1 ug/ml. detection:Chemiluminescence with an exposure time of 3 minutes.

Immunohistochemistry-Paraffin: API5 Antibody [NBP1-46223] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-API5 used at 1:200 (1 ug/ml) Detection: DAB

Immunohistochemistry-Paraffin: API5 Antibody [NBP1-46223] - Detection of human API5 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-API5 (NBP1-46223). Detection: DAB

Immunoprecipitation: API5 Antibody [NBP1-46223] - Detection of human API5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat, cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-API5 antibody NBP1-46223 (lot NBP1-46223-2) used for IP at 3 ug per reaction. API5 was also immunoprecipitated by a previous lot of this antibody (lot NBP1-46223-1) and another rabbit anti-API5 antibody. For blotting immunoprecipitated API5, NBP1-46223 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

• Reactivity: Hu, Mu, Ch, Pm-Or, Xp
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

API5 Antibody Summary

• Immunogen: The immunogen for this product maps to a region between residue 1 and 50 of human Apoptosis Inhibitor 5 using the numbering given in entry XP_508375.2 (GeneID 8539).
• Predicted Species: Orangutan (100%), Chicken (100%), Xenopus (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: API5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:500
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2-5 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for API5 Antibody

• AAC-11API5L1
• AAC11fibroblast growth factor 2-interacting factor 2
• Antiapoptosis clone 11 protein
• API-5
• apoptosis inhibitor 5
• Cell migration-inducing gene 8 protein
• Fibroblast growth factor 2-interacting factor
• FIF
• migration-inducing protein MIG8
• Protein XAGL

Background

Antiapoptotic factor that may have a role in protein assembly. Negatively regulates ACIN1. By binding to ACIN1, it suppresses ACIN1 cleavage from CASP3 and ACIN1-mediated DNA fragmentation. Also known to efficiently suppress E2F1-induced apoptosis. Its depletion enhances the cytotoxic action of the chemotherapeutic drugs

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: API5
• Entrez:
  • 8539(Human)
  • 11800(Mouse)
• Uniprot:
  • Q9BZZ5(Human)