APH1A Antibody (665404)

APH1A was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human APH1A Monoclonal Antibody (Catalog # MAB6908) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: IHC
• Clone: 665404
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

APH1A Antibody (665404) Summary

• Immunogen: Human APH1A synthetic peptide
  SSFERFEIFPKHVTDRSDARLQY
  Accession # Q96BI3
• Specificity: Detects human APH1A in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: APH1A
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for APH1A Antibody (665404)

• 6530402N02Rik
• anterior pharynx defective 1 homolog A (C. elegans)
• APH-1
• APH1A
• APH-1A
• aph-1alpha
• CGI-78
• gamma-secretase subunit APH-1A
• Presenilin-stabilization factor
• PSF

Background

Anterior pharynx-defective 1 (APH1A) is a widely expressed 36 kDa 7-transmembrane protein found in the endoplasmic reticulum and Golgi. APH1A is a component of the gamma secretase proteolytic complex which cleaves multiple substrates within their transmembrane domains. This activity liberates the intracellular domains of ErbB4, Neuregulin-1, and Notch receptors in addition to the A-beta peptide from the amyloid precursor protein (APP). Alternate splicing generates a short isoform of human APH1A that lacks 18 amino acids at the C-terminus. Within aa 58-69, human APH1A shares 100% aa sequence identity with mouse and rat APH1A.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: APH1A
• Uniprot:
  • Q96BI3()