Apc4 Antibody - BSA Free

Western Blot: Apc4 Antibody [NBP1-77378] - Analysis of APC4 in mouse liver tissue lysate with APC4 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry: Apc4 Antibody - BSA Free [NBP1-77378] - Immunohistochemistry of Apc4 in rat liver tissue with Apc4 antibody at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Apc4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Apc4 Antibody - BSA Free (NBP1-77378) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human APC4. The immunogen is located within amino acids 40 - 90 of APC4. Amino Acid Squence: WSFPPNENTGKEVT
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ANAPC4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  Apc4 Peptide (NBP1-77378PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Apc4 Antibody - BSA Free

• anaphase promoting complex subunit 4
• anaphase-promoting complex subunit 4
• APC4Cyclosome subunit 4

Background

Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC4 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC4, it is thought that APC4 associates with other APC/C components APC1, APC5, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ANAPC4
• Uniprot:
  • Q9UJX5(Human)