AP3S1 Antibody

Images

 
Western Blot: AP3S1 Antibody [NBP1-76588] - analysis of AP3S1 in mouse kidney tissue lysate with AP3S1 antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: AP3S1 Antibody [NBP1-76588] - Immunofluorescence of AP3S1 in human kidney tissue with AP3S1 antibody at 20 ug/mL.
Immunohistochemistry: AP3S1 Antibody [NBP1-76588] - Immunohistochemistry of AP3S1 in human kidney tissue with AP3S1 antibody at 2.5 ug/mL.

Product Details

Summary
Reactivity Hu, Mu, Rt, BvSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

AP3S1 Antibody Summary

Immunogen
Antibody was raised against a 19 amino acid synthetic peptide near the center of human AP3S1. The immunogen is located within the first 50 amino acids of AP3S1.
Specificity
At least three isoforms of AP3S1 are known to exist; this antibody will detect the two larger isoforms. AP3S1 antibody is predicted to not cross-react with other AP3 protein family members.
Predicted Species
Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
AP3S1
Purity
Peptide affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
Control Peptide

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for AP3S1 Antibody

  • Adapter-related protein complex 3 sigma-1 subunit
  • adaptor-related protein complex 3, sigma 1 subunit
  • AP-3 complex sigma-3A subunit
  • AP-3 complex subunit sigma-3A
  • CLAPS3AP-3 complex subunit sigma-1
  • Clathrin-associated/assembly/adapter protein, small 3
  • clathrin-associated/assembly/adaptor protein, small 3 (22kD)
  • Sigma3A
  • sigma-3A-adaptin
  • sigma3A-adaptin
  • Sigma-adaptin 3a

Background

AP3S1 belongs to the adaptor complexes 3 (AP3) small subunit family which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3 is an heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP3S1 Interacts with AGAP1 and may play an important role in carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol AP3S1
Uniprot