AP3S1 Antibody - BSA Free

Western Blot: AP3S1 Antibody [NBP1-76588] - analysis of AP3S1 in mouse kidney tissue lysate with AP3S1 antibody at (A) 1 and (B) 2 ug/mL .

Immunocytochemistry/ Immunofluorescence: AP3S1 Antibody - BSA Free [NBP1-76588] - Immunofluorescence of AP3S1 in human kidney tissue with AP3S1 antibody at 20 ug/mL.

Immunohistochemistry: AP3S1 Antibody - BSA Free [NBP1-76588] - Immunohistochemistry of AP3S1 in human kidney tissue with AP3S1 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

AP3S1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit AP3S1 Antibody - BSA Free (NBP1-76588) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-AP3S1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a 19 amino acid synthetic peptide near the center of human AP3S1. The immunogen is located within the first 50 amino acids of AP3S1. Amino Acid Squence: NHGKPRLSKFYQPYSEDTQ
• Specificity: At least three isoforms of AP3S1 are known to exist; this antibody will detect the two larger isoforms. AP3S1 antibody is predicted to not cross-react with other AP3 protein family members.
• Predicted Species: Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: AP3S1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1-2 ug/ml
• Control Peptide:

  AP3S1 Peptide (NBP1-76588PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for AP3S1 Antibody - BSA Free

• Adapter-related protein complex 3 sigma-1 subunit
• adaptor-related protein complex 3, sigma 1 subunit
• AP-3 complex sigma-3A subunit
• AP-3 complex subunit sigma-3A
• CLAPS3AP-3 complex subunit sigma-1
• Clathrin-associated/assembly/adapter protein, small 3
• clathrin-associated/assembly/adaptor protein, small 3 (22kD)
• Sigma3A
• sigma-3A-adaptin
• sigma3A-adaptin
• Sigma-adaptin 3a

Background

AP3S1 belongs to the adaptor complexes 3 (AP3) small subunit family which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. AP3 is an heterotetramer composed of two large adaptins (AP3D1, AP3B1 or AP3B2), a medium adaptin (AP3M1 or AP3M2) and a small adaptin (APS1 or AP3S2). AP3S1 Interacts with AGAP1 and may play an important role in carcinoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AP3S1 Antibody (NBP1-76588)(1)

Publication using NBP1-76588

• VC Cabana, AY Bouchard, AM Sénécal, K Ghilarducc, S Kourrich, L Cappadocia, MP Lussier RNF13 Dileucine Motif Variants L311S and L312P Interfere with Endosomal Localization and AP-3 Complex Association Cells, 2021-11-06;10(11):. 2021-11-06 [PMID: 34831286]

Bioinformatics

• Gene Symbol: AP3S1
• Uniprot:
  • NP_001275(Human)