AP-2 epsilon/TFAP2E Antibody [Unconjugated]

Western blot shows lysates of A431 human epithelial carcinoma cell line and human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human AP-2e Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for AP-2e at approximately 48 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

AP-2 epsilon/TFAP2E Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human AP-2 epsilon
  Ala123-Val217
  Accession # Q6VUC0
• Specificity: Detects human AP-2 epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross‑reactivity with recombinant human (rh) AP‑2 alpha , rhAP‑2 beta , rhAP‑2δ, and rhAP‑2 gamma is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TFAP2E
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for AP-2 epsilon/TFAP2E Antibody [Unconjugated]

• Activating enhancer-binding protein 2-epsilon
• adaptor-related protein complex 2, epsilon subunit
• AP2 epsilon
• AP-2 epsilon
• AP2E
• AP2-epsilon
• MGC49007
• TFAP2E
• transcription factor AP-2 epsilon (activating enhancer binding protein 2epsilon)
• transcription factor AP-2-epsilon

Background

AP-2 epsilon (Activating protein 2 epsilon) is a 48 kDa member of the AP-2 transcription factor family. It is found in embryonic olfactory bulb mitral cells and adult keratinocytes and chondrocytes. In the nucleus, it presumably forms homodimers and heterodimers with other AP‑2 family members. Human AP‑2 epsilon is 442 amino acids in length. It contains a Gln/Pro-rich transactivation domain (aa 22‑122), a DNA binding domain (aa 216‑286) and an AP‑2, helix‑span‑helix dimerization region (aa 287‑417). There is one potential alternate start site at Met09. Over aa 123‑217, human AP‑2 epsilon shares 86% aa identity with mouse AP‑2 epsilon .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for AP-2 epsilon/TFAP2E Antibody (AF5060)(4)

Publications using AF5060

• Dietz, A;Senf, K;Neuhaus, EM; Stem cell expressed CXCR4 regulates tissue composition in the vomeronasal organ Journal of cell science 2024-12-06 [PMID: 39639824] (Immunohistochemistry, Mouse)
• Amato, E;Taroc, EZM;Forni, PE; Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development bioRxiv : the preprint server for biology 2023-09-03 [PMID: 37693459] (IHC, Mouse)
• Jennifer M Lin, Tyler A Mitchell, Megan Rothstein, Alison Pehl, Ed Zandro M Taroc, Raghu R Katreddi et al. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2 epsilon in vomeronasal sensory neurons eLife 2022-09-16 [PMID: 36111787]
• Raghu Ram Katreddi, Ed Zandro M. Taroc, Sawyer M. Hicks, Jennifer M. Lin, Shuting Liu, Mengqing Xiang et al. Notch signaling determines cell-fate specification of the two main types of vomeronasal neurons of rodents Development 2022-07-01 [PMID: 35781337]

Bioinformatics

• Gene Symbol: TFAP2E
• Uniprot:
  • Q6VUC0()